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dc.contributor.authorWilson, Marcus T.en_NZ
dc.contributor.authorLin, Oliver D.en_NZ
dc.contributor.authorVoss, L.J.en_NZ
dc.contributor.authorJones, K.en_NZ
dc.date.accessioned2015-12-02T00:50:48Z
dc.date.available2015en_NZ
dc.date.available2015-12-02T00:50:48Z
dc.date.issued2015en_NZ
dc.identifier.citationWilson, M. T., Lin, O. D., Voss, L. J., & Jones, K. (2015). Measuring the bulk impedance of brain tissue in vitro. In Metrology Society of Australasia Conference, MSA Conference, Queenstown, New Zealand, October 14-16, 2015en
dc.identifier.urihttps://hdl.handle.net/10289/9781
dc.description.abstractTheoretical and numerical models of brain activity suggest a link between seizures and electrical connectivity. We have therefore been motivated to measure electrical conductivity in brain tissue. Such measurements in vitro are difficult; it is necessary to use a conductive inorganic salt solution, artificial cerebrospinal fluid (ACSF), to keep the tissue alive. We have attempted to provide a robust method to make such measurements. Mouse brain tissue was sliced (400 μm) using established methods. Half the slices were placed in standard ACSF; half were placed in ACSF devoid of magnesium ions. The latter case promotes seizure activity. Electrical activity was measured with a tungsten electrode at various places on the slices. Sixty-nine samples of cortex (2 mm × 2 mm) were cut with a razor. Their areas were measured with a calibrated microscope. Each sample was placed between two flat Ag/AgCl electrodes in a Perspex sandwich. Excess ACSF was removed with filter paper. The impedance was measured at 25°C from 20 Hz to 2 MHz with an Agilent E4980A four-point impedance meter in a shielded room, using a low current. Between 1 kHz and 100 kHz the conductivity was approximately 0.2 S m⁻¹; outside this range dispersion occurred. Samples prepared in the magnesium-free ACSF had a conductivity about 10% lower. The Cole-Cole model of conductivity was fitted. There were few significant differences between the parameters for the different groups measured.en_NZ
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.rights© This work is licensed under a Creative Commons Attribution - No Derivatives 4.0 International License.
dc.sourceMetrology Society of Australasia Conferenceen_NZ
dc.titleMeasuring the bulk impedance of brain tissue in vitroen_NZ
dc.typeConference Contribution
dc.relation.isPartOfMetrology Society of Australasia Conferenceen_NZ
pubs.elements-id134058
pubs.finish-date2015-10-16en_NZ
pubs.publisher-urlhttp://www.metrology.asn.au/msaconnected/conference-homeen_NZ
pubs.start-date2015-10-14en_NZ


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