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Maintenance of cyanotoxin production by cryopreserved cyanobacteria in the New Zealand culture collection

Abstract
A culture collection of freshwater planktonic and benthic cyanobacteria collected from sites across New Zealand has been established at the Cawthron Institute, Nelson, New Zealand. Limited resources led to uncertainty regarding the long-term maintenance of this collection. The present study demonstrates cryopreservation to be a viable method for long-term storage of cyanobacteria. Seventeen of 20 strains evaluated were successfully cryopreserved using the permeating cryopreservation agent dimethyl sulfoxide (Me2 SO), at a final concentration of 15% (v/v). Cyanotoxin analysis was undertaken on selected strains known to produce microcystins, nodularin, anatoxin-a, and saxitoxins. All strains retained their ability to produce these toxins following cryopreservation.
Type
Journal Article
Type of thesis
Series
Citation
Wood, S. A., Rhodes, L. L., Adams, S. L., Adamson, J. E., Smith, K. F., Smith, J. F., Tervit, H. R. & Cary, S. C. (2008). Maintenance of cyanotoxin production by cryopreserved cyanobacteria in the New Zealand culture collection. New Zealand Journal of Marine and Freshwater Research, 42(3), 277- 283.
Date
2008
Publisher
RSNZ Publishing
Degree
Supervisors
Rights