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Cryosolvents useful for protein and enzyme studies below −100°C

Abstract
For the study of protein structure, dynamics, and function, at very low temperatures it is desirable to use cryosolvents that resist phase separation and crystallisation. We have examined these properties in a variety of cryosolvents. Using visual and X-ray diffraction criteria, methanol:ethanediol (70%:10%), methanol:glycerol (70%:10%), acetone:methoxyethanol:ethanediol (35%:35%:10%), dimethylformamide:ethanediol (70%:10%), dimethylformamide (80%), methoxyethanol (80%), and methoxyethanol:ethanediol (70%:10%) were all found to be free of phase-changes down to at least −160°C. The least viscous of these, methanol:ethanediol (70%:10%), was miscible down to −125°C and showed no exo or endothermic transitions when examined using DSC. It is therefore potentially particularly suitable for very low temperature cryoenzymology.
Type
Journal Article
Type of thesis
Series
Citation
Reat, V., Finney, J.L., Steer, A., Roberts, M.A., Smith, J., …, Daniel, R. M. (2000). Cryosolvents useful for protein and enzyme studies below −100°C. Journal of Biochemical and Biophysical Methods, 42(3), 97-103.
Date
2000
Publisher
Elsevier
Degree
Supervisors
Rights