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Determination of total potentially available nucleosides in bovine milk

Abstract
Bovine colostrum and milk samples were collected from two herds over the course of the first month post-partum, pooled for each herd by stage of lactation and total potentially available nucleosides were determined. Sample analysis consisted of parallel enzymatic treatments, phenylboronate clean-up, and liquid chromatography to quantify contributions of nucleosides, monomeric nucleotides, nucleotide adducts, and polymeric nucleotides to the available nucleosides pool. Bovine colostrum contained high levels of nucleosides and monomeric nucleotides, which rapidly decreased as lactation progressed into transitional milk. Mature milk was relatively consistent in nucleoside and monomeric nucleotide concentrations from approximately the tenth day post-partum. Differences in concentrations between summer-milk and winter-milk herds were largely attributable to variability in uridine and monomeric nucleotide concentrations.
Type
Journal Article
Type of thesis
Series
Citation
Gill, B.D., Indyk, H.E. & Manley-Harris, M. (2010). Determination of total potentially available nucleosides in bovine milk. International Dairy Journal, 21(1), 34-41.
Date
2010
Publisher
Elsevier
Degree
Supervisors
Rights
This is an author’s accepted version of an article published in the journal: International Dairy Journal. © 2010 Elsevier.