Xie, Congmin ZoeHealy, Terry R.Robinson, PeterStewart, Kevin2013-10-252013-10-252010Xie, C., Z., Healy, T. R., Robinson, P., & Stewart, K. (2010). Determination of EDTA in dairy wastewater and adjacent surface water. International Journal of Civil and Environmental Engineering, 2(1), 44-48.https://hdl.handle.net/10289/8111An HPLC-UV analytical method was developed to determine ethylenediaminetetraacetic acid (EDTA) in dairy wastewater and surface water. The optimizing separation was achieved by reversed–phase ion-pair liquid chromatography on a C18 column using methanol as mobile phase solvent, tetrabutylammonium bromide as the ion-pair reagent in pH 3.3 formate buffer solution at a flow rate of 0.9 mL min⁻¹ with a UV detector at 265 nm. No interference of Ca, Mg or NO₃⁻ was detected. Method performance was evaluated in terms of linearity, repeatability and reproducibility. The method detection limit was 5 µg L⁻¹. The contents of EDTA in dairy effluents were 72 ~ 261 µg L⁻¹ at a large dairy site. A change of EDTA concentration was observed downstream of the dairy effluent discharge, but this was well under the predicted no effect concentration for aquatic ecosystemapplication/pdfenThis article has been published in the journal: International Journal of Civil and Environmental Engineering.dairy wastewaterEDTAHPLCsurface waterJournal Article