Balog, ErikaBecker, TorstenOettl, MartinLechner, RuepDaniel, Roy M.Finney, John L.Smith, Jeremy C.2010-04-142010-04-142004Balog, E., Becker, T., Oettl, M., Lechner, R., Daniel, R. M., Finney, J. & Smith, J. C. (2004). Direct determination of vibrational density of states change on ligand binding to a protein. Physical Review letters, 93(2), 028103.https://hdl.handle.net/10289/3796The change in the vibrational density of states of a protein (dihydrofolate reductase) on binding a ligand (methotrexate) is determined using inelastic neutron scattering. The vibrations of the complex soften significantly relative to the unbound protein. The resulting free-energy change, which is directly determined by the density of states change, is found to contribute significantly to the binding equilibrium.application/pdfenThis article has been published in the journal: Physical Review letters. ©2004 The American Physical Society.proteinvibrational densityJournal Article10.1103/PhysRevLett.93.028103