Ting, Wei-JiaHuang, Chun-MingNair, Giridhar R.Wu, Wen-Teng2010-07-182010-07-182008Ting, W.-J., Huang, C.-M., Nair, G.R. & Wu, W.-T. (2008). An enzymatic/acid-catalyzed hybrid process for biodiesel production from soybean oil. Journal of the Chinese Institute of Chemical Engineers, 39(3), 203-210.https://hdl.handle.net/10289/4157The present study is aimed at developing an enzymatic/acid-catalyzed hybrid process for biodiesel production using soybean oil as feedstock. In the enzymatic hydrolysis, 88% of the oil taken initially was hydrolyzed by binary immobilized lipase after 5 h under optimal conditions. The hydrolysate was further used in acid-catalyzed esterification for biodiesel production and the effects of temperature, catalyst concentration, feedstock to methanol molar ratio, and reaction time on biodiesel conversion were investigated. By using a feedstock to methanol molar ratio of 1:15 and a sulfuric acid concentration of 2.5%, a biodiesel conversion of 99% was obtained after 12 h of reaction at 50 °C. The biodiesel produced by this process met the American Society for Testing and Materials (ASTM) standard. This hybrid process may open a way for biodiesel production using unrefined and used oil as feedstock.enacid catalystbinary immobilized lipasebiodieselhybrid processJournal Article10.1016/j.jcice.2008.01.004