Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome

Health, CarolineHu, Xiao PingCary, S. CraigCowan, Don A.2011-06-072011-06-072009Health, C., Hu, X.P., Cary, S.C. & Cowan, D. (2009). Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome. Applied and Environmental Microbiology, 77, 1153-1161.https://hdl.handle.net/10289/5382A novel esterase was identified through functional screening of an Antarctic desert soil metagenomic library in E. coli. The 297-amino acid sequence had only low (< 29 %) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7-54 °C and at alkaline pH levels, and is a potential candidate for industrial application.enAntarctic desertsoil metagenomeIsolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenomeJournal Article10.1128/AEM.02597-08