Adams, Christopher. J.Boult, Cherie H.Deadman, Benjamin J.Farr, Judie M.Grainger, Megan N.C.Manley-Harris, MerilynSnow, Melanie J.2013-11-222013-11-222008-032008Adams, C. J., Boult, C. H., Deadman, B. J., Farr, J. M., Grainger, M. N. C., Manley-Harris, M., & Snow, M. J. (2008). Isolation by HPLC and characterisation of the bioactive fraction of New Zealand manuka (Leptospermum scoparium) honey. Carbohydrate Research, 343(4), 651-659.https://hdl.handle.net/10289/8221Using HPLC a fraction of New Zealand manuka honey has been isolated, which gives rise to the non-peroxide antibacterial activity. This fraction proved to be methylglyoxal, a highly reactive precursor in the formation of advanced glycation endproducts (AGEs). Methylglyoxal concentrations in 49 manuka and 34 non-manuka honey samples were determined using a direct detection method and compared with values obtained using standard o-phenylenediamine derivatisation. Concentrations obtained using both the methods were similar and varied from 38 to 828 mg/kg.enNew Zealand manuka honeybioactive fractionmethylglyoxalJournal Article10.1016/j.carres.2007.12.011