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Publication Ngā hau pūkeri e tīhaehae nei i ā tātou tikanga Māori(Thesis, The University of Waikato, 2003)Ko te wherawhera a tēnei tuhinga he titiro ki ngā io mai i ngā tikanga o te ao Māori, ā, ki ngā hau kino e whakataurekareka ana i ā tātou tikanga Māori. Kei roto i ngā iwi katoa o te ao ā rātau tikanga, engari, ko ngā whakamāramatanga o aua tikanga kei te mau ruhiruhi noa i roto i ngā hinengaro o ngā tāngata o tēnā iwi, tēnā iwi, ā, kua ngaro katoa atu rānei. Nā reira, ko tāku he rapu i aua māramatanga kei muri i ngā tikanga Māori, ā, he rapu anō hoki i ngā tāmitanga, i ngā pēhitanga a tauiwi mā, ko te mutunga ka whakapōrearea i ā tātou tikanga Māori. Ko te aronga rangahau mō tēnei kaupapa he whai i ngā tuhinga me ngā kōrero i ahu mai i te ao Māori me te ao Pākehā. Ko ngā rauemi nei i ahu mai i ngā kohikohinga kōrero tawhito me ngā tuhinga a te hunga o roto i ngā tau kātahi anō ka pahure ake. Ko ngā kōrero tawhito nei hei whakaata ake i te huringa o te ao, ā, ka tīkina atu aua kōrero hei ārahi i te hunga ora kia mau tonu ai ā tātou tikanga Māori. Ki te whai haere i tēnei momo kaupapa, me mātua whai rawa i ngā kōrero ā-waha hei whakaatu mai, ina kāore hoki i āta tuhia whānuihia ngā kōrero i maringi mai i te hinengaro o te hunga whakautu pātai. Ko ngā kohinga kōrero ā-waha hei tohu i ngā tikanga me ngā whakapono ake i pā mai ki a rātou i te wā e tipu ake ana. Ko ētahi o ngā kaikōrero i tipu ake i roto i te tūturutanga o te ao Māori, arā, i tipu ake i ngā rekereke o ō rātou kuia, koroua, ā, ko ētahi ahakoa he Māori, i tipu ake anō i roto i te reo me ētahi o ngā tikanga Māori, ko ētahi anō i noho hoki i te tāone. Ko ngā kaupapa ka whakaarahia ake e tēnei rangahau ko ngā tikanga e hāngai pū ana, e whakamahia ana, e kitea ana, e whakaatu ana anō hoki i ngā whakapono o te Māori. Kāti, ka tirohia ētahi tikanga i waenga i te whānau, ētahi atu tikanga hāngai pū ana ki te manaaki tangata i runga marae me te whakaaro ake ki ngā tohu e tika ana ki te whakanui manuhiri. He roa te Māori e noho ana i te ao Pākehā, mai i tō rātou taenga mai i te rau tau tekau mā waru. Ko te nuinga o tātau kua roa kē te wehenga mai i te ao tūturu ake o te Māori. Ko ētahi tāngata e pupuri tonu ana i ētahi o ā tātou tikanga, ko ētahi kāhore noa e mōhio ana. Tēnā pea, ko ngā whakatūpato i mau, engari, ko ngā whakamārama kei te ngaro. Kāti, ko te tūmanako mō tēnei tuhingaroa ko te whakahoutia anō o te here ki ā tātou tikanga. Ka āhei hoki te hunga e ngākaunui ana ki te kaupapa nei ki te whai whakaaro, te whai māramatanga. Ko tēnei tuhinga he tauira hei whakaatu atu he mahi pakeke te hopu, te tiaki, te manaaki me te ako hoki i ngā mātauranga o tuawhakarere. He pēnei anō ēnei tikanga mō nāianei, he toimaha tonu te kawe, te whakaū. Heoi, ka whakatakia mai ngā kōrero i te orokohanga o te ao hei raranga ake hei whāriki mō te tikanga Māori. Tuatahi, ka tirohia ētahi o ngā whenu o tēnei mahi whata, arā, te ahunga mai o te tikanga Māori me ngā tikanga huhua i whakatauiratia mai e ngā atua Māori. Whai muri atu kei te upoko tuarua, ka whakaraupapahia mai ngā kōrero a te hunga whakautu pātai kia āta kitea ai ō rātou whakaaro mō te tikanga Māori. Ka hāngai pū tonu tēnei titiro ki ngā momo pātai, ā, ka tirohia hoki ngā whakahokinga a taua hunga nā. Kei te upoko tuatoru ka huri te titiro ki ngā hau pūkeri e tūkino nei i ā tātou tikanga Māori. Tuatahi, ka horahia ngā kōrero, ngā tikanga mō te āhua o te noho a te Māori o mua, kia āta kitea ai i pēhea te āhua o tōna whakahaere i tōna ao o mua. Kātahi, ka āta tirohia ētahi ture Pākehā e tīhaehae nei i ā tātou tikanga, pēnei i te ngaro haere o te reo Māori, te haehae haere o ā tātou tikanga Māori. Ka mutu, ka huri ake ki te whai whakaaro ki ētahi o ngā raru i pā mai ki te tikanga Māori i te neketanga o te Māori ki te tāone noho ai, mahi ai. Kei te upoko tuawhā ētahi tauira mō ngā tūmomo tikanga e mahia ana e te Māori i waenganui i tōna whānau. Ka tirohia te ringa tapu o te tikanga hei whakaohooho ake i te hinengaro, hei whakatakoto māramatanga mō aua tikanga hoki. Kei te upoko tuarima te tirohanga ki ngā momo tikanga i runga marae, me ngā momo tohu Māori e whakaatu mai ana i te rangatiratanga o te iwi, pēnei i ngā tohu kino, i ngā tohu ora rānei. Ka tirohia ētahi whakamāramatanga mō te āhua o te noho piri o te Māori ki tōna taiao, he aha ngā kōrero a te taiao ki te Māori, he aha hoki ngā kōrero a ngā kararehe o te taiao. Hei whakakapi ake i te tuhinga nei ko te whakarāpopototanga. I konei ka titiro whakamuri ki ngā āhuatanga kua ara ake. Ka whakaraupapahia ngā whakakitenga me ngā whakaputanga hei whakaohooho, hei whakaarotanga ake mō te ao kei mua e pā ana ki ā tātou tikanga Māori.Publication Bacteriological control food equipment by cleaning systems(Thesis, The University of Waikato, 2024)This study was undertaken to examine the performance of cleaning systems applied to food equipment. The contribution by the components of those systems to the performance of the total system was determined. A cleaning simulator was constructed in which test pieces in 4 test cells were subjected to 25 to 35 cycles, each of 12h. Each cycle the test pieces were: soiled for 45 min. in pasteurised milk (at 30°c) dosed with 10⁶ bacteria/ml of Streptococcus faecalis, Escherichia coli or Enterobacter aerogenes; serially treated with the rinses, detergents and sanitizers of the experimental cleaning system; and left for 9.5h intercycle (rest) period. The changes in the soil mass with time on stainless steel, rubber and glass test pieces were determined gravimetrically. The changes in the numbers of the dosed and non-dosed organisms on the surfaces, after components of the cleaning system had been applied, were determined by a swab-recovery/ plate-counting system. The surfaces were also examined by scanning electron microscopy. Four experiments were performed using this technique. A field experiment examined the performance of 5 cleaning systems over experimental periods of 5 weeks on 15 milking machines. The nature of the changes in the performance of the cleaning system over time were determined. Both detergents and sanitizers could, under varying conditions, contribute significantly to the bacteriological performance achieved by the system. A sanitizer applied for the complete intercycle period was more efficient than those applied for short contact-times. A post-wash sanitizer was slightly more efficient than a sanitizer applied before milking. The cycle of bacterial deposition onto the equipment surfaces, attachment, depletion by the cleaning system, growth during the intercycle period, and subsequent contamination of the milk at the next milking was studied. All three dose organisms attached actively, and were then largely removed by the cleaning system. Despite there being a large amount of soil on the surfaces of the test pieces, the intercycle conditions (30°c at up to 90% RH for 9.5h) were insufficient for growth. The effect of the amount of soil on the surface on the ability of bacteria to survive the application of a system or system component was examined. The minimum soil levels necessary for an organism to survive a treatment (Total Effect Maximum) and the minimum soil necessary for all organisms to survive a treatment (Zero Effect Minimum) were determined for systems and system components. The data was used to discuss the principles to be followed in designing a cleaning system. The principles were applied to the design of a system to clean a milking machine with cold solutions by soak techniques.Publication The germination of seeds(Thesis, The University of Waikato, 1971)Literature Reviews of seed dormancy, the germination of seeds (restricted as closely as possible to the period actually preceding radicle protrusion) and the effects of gamma radiation are included in this thesis. Tritiated water studies of heavily irradiated Sinapis alba seeds showed that these seeds can carry out active metabolism, leading to the formation of tritiated gamma-aminobutyric acid (GABA), alanine, malic acid and citric acid. The compounds which were not labelled by tritiated water in heavily irradiated seeds but which were labelled in non-irradiated seeds were the following: lactic acid, glutamine, sucrose, glutamic acid, aspartic acid and sugar phosphates. Difficulty was experienced in enabling sufficient C-14 substrate to enter the seeds for studying the metabolism of these compounds. The seeds were therefore ground in liquid air prior to the C14-tracer studies. The metabolism of this powder was examined using tritiated water. Over short time intervals this metabolism was similar to that of whole seeds except for the heavy labelling of lipid material which was shown to be ninhydrin positive. At longer time intervals this powder did not develop the complex patterns typical of whole seeds. The metabolites lactic acid, glutamine, sucrose and sugar phosphates were not labelled during 24 hours imbibition in tritiated water, of the Liquid Air Powder (LAP) but were labelled on imbibition of whole seeds. Imbibition of the LAP of both irradiated and non-irradiated seeds with the C-14 substrates gave similar distributions of radioactivity. Aspartate-U-C14 was not rapidly metabolised within 4 hours. In non-irradiated seeds traces of a ninhydrin positive compound (U1) postulated to be succinylarginine were found after 4 hours. Incubation with GABA-1-C14 led to labelled flavonoids within one hour and to labelled alanine and an unknown (U2) within 4 hours. The metabolism of glutamate-U-C14 rapidly led to the formation of GABA at short time intervals and at longer time intervals (4 hours) to labelled GABA, alanine, and two unknowns called “YPN” and U3. In the irradiated seeds labelled lipid was also formed. The unknowns U2 and U3 are postulated to be the same ninhydrin positive compound, possibly a peptide. The transaminases, glutamate-oxaloacetate and glutamate-pyruvate were found to be active in enzyme extractions of dry seeds. Evidence showing a reduction in the activity of transaminases with time of imbibition is also reported. Paper chromatographic analyses of seed metabolism, including a novel method developed for a rapid enzyme assay are included. These results were similar to those obtained using C-14 substrates. In addition conversion of GABA and glutamate to an unknown called UVGB was found. The metabolism of aspartate led to alanine and of alanine, to GABA. The limited metabolism displayed by heavily irradiated seeds immediately after irradiation did not lead to the formation of a seedling for those Sinapis alba seeds which had received a dose of 2.98 Mrep or more. The germination of a very large range of seed species was examined after receiving chronic doses of irradiation. Only parsnip seeds would not germinate after receiving a dose of 0.5 Mrads. Most seeds showed similar responses to that for mustard seeds. One group of irradiated mustard seeds were left in a drawer in the laboratory. These seeds showed complete recovery in both the ability to germinate and in their labelling patterns when imbibed with tritiated water after less than 6 months storage. A similar set of seeds stored over silica gel did not display any recovery. A large experiment was undertaken in which seeds of many species were examined after various periods of storage at fixed or room humidities. Some recovery was observed although not of the same order as that found earlier. Storage at very high humidities led to loss of the ability to germinate. The effects of various solutions were also examined and once again a limited amount of recovery was observed. It is therefore postulated that some other factor, not a fixed relative humidity, was responsible for the dramatic recovery of the first group of mustard seeds. This could be a short period at high relative humidities followed by a longer period at low humidities or possibly some gas in the atmosphere of the laboratory at that time. Plants were grown from the fully recovered mustard seeds. The greater weight and height of these plants compared to those from non-irradiated seeds were shown to be statistically significant.Publication Laser light scattering as a probe of bovine sperm motility(Thesis, The University of Waikato, 1980)Laser light scattering measurements of sperm motility have traditionally assumed spermatozoa to behave as point scatterers at small forward scattering angles, and motile cells to exhibit an isotropic distribution of translation vectors. Such assumptions have lead to interpretations of fluctuations in the detected optical field as arising from Doppler-beats. Experimental and theoretical results presented in this thesis show that for bull spermatozoa, the large dimensions (>> λ) and slab-like conformation of the rotating head result in large intensity fluctuations at the photodetector which call into question the role of coherence and render the Doppler-beat interpretation untenable. A sharply peaked scattering lobe (width ~ 20°) lying in the equatorial plane of the head is shown to give an intensity peak when the normal to the head plane becomes aligned with the scattering vector. Consequently only those motile cells swimming with appropriately aligned translation vectors contribute to the detected signal. Immotile cells, which are shown to exhibit geotaxis, become visible only to a detector aligned in the horizontal plane. A marked concentration dependence in the ratio of motile and immotile autocorrelation components is modelled on the basis of hydrodynamic interactions between motile and immotile fractions, which result in immotile cells being reoriented outside the range of visible alignments. A complex opto-dynamic system is defined by a series of experiments which demonstrate that the traditional laser light scattering geometry gives only an empirical measure of the percent motile spermatozoa but may be used to obtain an absolute measurement of the head rotation rate. Discussion is given to variations in the scattering geometry and sample chamber design which are more appropriate to the phenomenology within the system.Publication Catecholamine physiology in the sheep(Thesis, The University of Waikato, 1979)A study of catecholamine physiology in the sheep has been carried out with emphasis on the measurement of plasma catecholamine levels and their interactions with lactic acid and cortisol. The plasma dynamics of the catecholamines were first examined under laboratory conditions followed by an assessment of sympathetic responses to a variety of environmental stimuli. Catecholamine levels were monitored in the plasma of sheep during and after 90 minute infusions of 1-dopa, dopamine, noradrenaline and adrenaline. A 2-compartmental pharmacokinetic model of 1-dopa plasma dynamics was constructed and the half-lives of the fast and slow decay curves determined. Estimation of the half-lives of dopamine, noradrenaline and adrenaline was prevented by a period of increased endogenous secretion that occurred on the termination of the infusions. A hypothesis based on the hypotension developed by sheep on the arrest of catecholamine infusions of this nature was developed to explain the adrenergic activity observed. Lactic acid levels rose markedly during the adrenaline infusions with a much smaller response during noradrenaline infusions while a biphasic cortisol response to both the noradrenaline and adrenaline infusions was observed. A set of blood samples was collected from each of 6 cannulated lambs at various stages of a journey from the farm to the abattoir. Catecholamine levels remained consistently elevated throughout the experiment before rising to a very high peak upon electrical stunning prior to slaughter. Plasma lactic acid peaks were associated with mustering and washing while cortisol remained high throughout the experiment until penning at the abattoir when it fell sharply to baseline levels. Subsequently it rose overnight. The physiological basis of these observations is discussed in relation to the transport of stock and the use of these blood substances in assessing the reactions of domestic animals to their environment. The massive catecholamine release in response to electrical stunning was further studied in a series of experiments. Significant differences between lamb mobs slaughtered at an abattoir appeared but a relationship was not found between the catecholamine plasma concentrations and the haemorrhagic lesion known as “blood splash” caused by electrical stunning. Short-term stressors encountered by the lambs at the abattoir had no effect on the blood splash incidence but some evidence suggested a link with the previous behavioural and handling conditions of slaughtered lambs. Both the α-adrenergic and the β-adrenergic blocking drugs phentolamine and propranolol were effective in suppressing blood splash and the physiological basis of this observation is considered. Some preliminary experiments to investigate catecholamine involvement in the neurological disease of ryegrass staggers in sheep are described together with a description of the intense ingestive behaviour and the possible taming effects induced by the central dopaminergic receptor blocker haloperidol. The application of these and other results to future agricultural research programmes is considered in the light of recent developments in the understanding of the multiple roles of these substances in mammalian physiology.
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