Isolation from Rhizobium of factors which transfer electrons to soybean nitrogenase

Abstract

Rhizobium japonicum grown anaerobically with nitrate produced several factors capable of transferring electrons from spinach chloroplasts lacking photosystem II to soybean root nodule nitrogenase. The same organism grown aerobically produced no factors with detectable activity. It is concluded that the bacterium is potentially capable of producing these proteins in the low oxygen conditions of the leguminous root nodule.