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dc.contributor.authorBertone, Edoardoen_NZ
dc.contributor.authorChuang, Annen_NZ
dc.contributor.authorBurford, Michele A.en_NZ
dc.contributor.authorHamilton, David P.en_NZ
dc.coverage.spatialNetherlandsen_NZ
dc.date.accessioned2020-07-30T21:50:59Z
dc.date.available2020-07-30T21:50:59Z
dc.date.issued2019en_NZ
dc.identifier.citationBertone, E., Chuang, A., Burford, M. A., & Hamilton, D. P. (2019). In-situ fluorescence monitoring of cyanobacteria: Laboratory-based quantification of species-specific measurement accuracy. Harmful Algae, 87, 101625. https://doi.org/10.1016/j.hal.2019.101625en
dc.identifier.urihttps://hdl.handle.net/10289/13702
dc.description.abstractIn recent years, in-situ fluorometers have been extensively deployed to monitor cyanobacteria in near real-time. Acceptable accuracy can be achieved between measured pigments and cyanobacteria biovolume provided the cyanobacteria species are known. However, cellular photosynthetic pigment content and measurement interferences are site and species specific and can dramatically affect sensor reliability. We quantified the accuracy of an in-situ fluorometer compared with traditional methods using mono- and mixed cultures of four different cyanobacterial species. We found: (1) lower pigment content in cultures in stationary phase, (2) higher precision with the sensor compared to traditional pigment quantification methods of measuring phycocyanin and chlorophyll a, (3) species-specific relationships between sensor readings and measurements related to biovolume, (4) overestimation of pigments in mixed compared with mono cultures, (5) dissolved organic matter causing a loss in signal proportional to its degree of aromaticity, and (6) potential to quantify the degree of cell lysis with a fluorescent dissolved organic matter sensor. This study has provided important new information on the strengths and limitations of fluorescence sensors. The sensor readings can provide accurate biovolume quantification and species determination for a number of bloom-forming species when sensors are properly compensated and calibrated.en_NZ
dc.language.isoenen_NZ
dc.publisherElsevier
dc.rightsThis is an author’s accepted version of an article published in the journal: Harmful Algae. © 2019 Elsevier.
dc.subjectCyanobacteriaen_NZ
dc.subjectFluorescenceen_NZ
dc.subjectRemote sensingen_NZ
dc.subjectWater qualityen_NZ
dc.subjectWater resources managementen_NZ
dc.subjectChlorophyll Aen_NZ
dc.subjectCyanobacteriaen_NZ
dc.subjectEnvironmental Monitoringen_NZ
dc.subjectPhycocyaninen_NZ
dc.subjectReproducibility of Resultsen_NZ
dc.titleIn-situ fluorescence monitoring of cyanobacteria: Laboratory-based quantification of species-specific measurement accuracy.en_NZ
dc.typeJournal Article
dc.identifier.doi10.1016/j.hal.2019.101625en_NZ
dc.relation.isPartOfHarmful Algaeen_NZ
pubs.begin-page101625
pubs.elements-id238658
pubs.publication-statusPublisheden_NZ
pubs.volume87en_NZ
dc.identifier.eissn1878-1470en_NZ


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