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Direct determination of vibrational density of states change on ligand binding to a protein
Abstract
The change in the vibrational density of states of a protein (dihydrofolate reductase) on binding a ligand (methotrexate) is determined using inelastic neutron scattering. The vibrations of the complex soften significantly relative to the unbound protein. The resulting free-energy change, which is directly determined by the density of states change, is found to contribute significantly to the binding equilibrium.
Type
Journal Article
Type of thesis
Series
Citation
Balog, E., Becker, T., Oettl, M., Lechner, R., Daniel, R. M., Finney, J. & Smith, J. C. (2004). Direct determination of vibrational density of states change on ligand binding to a protein. Physical Review letters, 93(2), 028103.
Date
2004
Publisher
American Physical Society
Degree
Supervisors
Rights
This article has been published in the journal: Physical Review letters. ©2004 The American Physical Society.