Publication: A general method for the synthesis of peptidyl substrate for proteolytic enzymes
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Abstract
The synthesis of an heterogeneous peptide substrate for the assay of proteases was carried out by cleaving a protein using a protease to generate small peptides which were then coupled to a chromophore, p-nitroaniline. The chromophoric peptide product could be used to assay for the protease which produced the original peptides.
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Whitmore, A., Daniel, R.M. & Petach, H.H. (1995). A general method for the synthesis of peptidyl substrate for proteolytic enzymes. Tetrahedron Letters, 36(3), 475-476.
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Elsevier