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dc.contributor.authorLee, P.T.en_NZ
dc.contributor.authorBird, Steveen_NZ
dc.contributor.authorZou, J.en_NZ
dc.contributor.authorMartin, S.A.M.en_NZ
dc.date.accessioned2017-05-18T00:04:22Z
dc.date.available2017en_NZ
dc.date.available2017-05-18T00:04:22Z
dc.date.issued2017en_NZ
dc.identifier.citationLee, P. T., Bird, S., Zou, J., & Martin, S. A. M. (2017). Phylogeny and expression analysis of C-reactive protein (CRP) and serum amyloid-P (SAP) like genes reveal two distinct groups in fish. Fish and Shellfish Immunology, 65, 42–51. https://doi.org/10.1016/j.fsi.2017.03.037en
dc.identifier.issn1050-4648en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/11061
dc.description.abstractThe acute phase response (APR) is an early innate immune function that is initiated by inflammatory signals, leading to the release of acute phase proteins to the bloodstream to re-establish homeostasis following microbial infection. In this study we analysed the Atlantic salmon (Salmo salar) whole-genome database and identified five C-reactive protein (CRP)/serum amyloid P component (SAP) like molecules namely CRP/SAP-1a, CRP/SAP-1b, CRP/SAP-1c, CRP/SAP-2 and CRP/SAP-3. These CRP/SAP genes formed two distinct sub-families, a universal group (group I) present in all vertebrates and a fish/amphibian specific group (group II). Salmon CRP/SAP-1a, CRP/SAP-1b and CRP/SAP-1c and CRP/SAP-2 belong to the group I family whilst salmon CRP/SAP-3 is a member of group II. Gene expression analysis showed that the salmon CRP/SAP-1a as well as serum amyloid A-5 (SAA-5), one of the major acute phase proteins, were significantly up-regulated by recombinant cytokines (rIL-1β and rIFNγ) in primary head kidney cells whilst the other four CRP/SAPs remained refractory. Furthermore, SAA-5 was produced as the main acute phase protein (APP) in Atlantic salmon challenged with Aeromonas salmonicida (aroA(-) strain) whilst salmon CRP/SAPs remained unaltered. Overall, these data illustrate the potential different functions of expanded salmon CRP/SAPs to their mammalian homologues.en_NZ
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherElsevieren_NZ
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S1050464817301651en_NZ
dc.rights© 2017 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
dc.subjectacute phase proteinen_NZ
dc.subjectC-reactive proteinen_NZ
dc.subjectserum amyloid-Pen_NZ
dc.subjectAtlantic salmonen_NZ
dc.subjectinnate immunityen_NZ
dc.subjectgene expressionen_NZ
dc.titlePhylogeny and expression analysis of C-reactive protein (CRP) and serum amyloid-P (SAP) like genes reveal two distinct groups in fishen_NZ
dc.typeJournal Article
dc.identifier.doi10.1016/j.fsi.2017.03.037en_NZ
dc.relation.isPartOfFish and Shellfish Immunologyen_NZ
pubs.begin-page42
pubs.elements-id193835
pubs.end-page51
pubs.organisational-group/Waikato
pubs.organisational-group/Waikato/2018 PBRF
pubs.organisational-group/Waikato/FSEN
pubs.organisational-group/Waikato/FSEN/2018 PBRF - FSEN
pubs.organisational-group/Waikato/FSEN/School of Science
pubs.organisational-group/Waikato/FSEN/School of Science/2018 PBRF - School of Science
pubs.volume65en_NZ
dc.identifier.eissn1095-9947en_NZ


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