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dc.contributor.authorMeinhold, Lars
dc.contributor.authorClement, David
dc.contributor.authorTehei, Moeava
dc.contributor.authorDaniel, Roy M.
dc.contributor.authorFinney, John L.
dc.contributor.authorSmith, Jeremy C.
dc.date.accessioned2008-11-07T00:38:19Z
dc.date.available2008-11-07T00:38:19Z
dc.date.issued2008-02
dc.identifier.citationMeinhold, L., Clement, D., Tehei, M., Daniel, R., Finney, J. L. & Smith, J.C. (2008). Protein dynamics and stability: The distribution of atomic fluctuations in thermophilic and mesophilic dihydrofolate reductase derived using elastic incoherent neutron scattering. Biophysical Journal, 94, 4812- 4818.en_US
dc.identifier.issn1542-0086
dc.identifier.urihttps://hdl.handle.net/10289/1289
dc.description.abstractThe temperature dependence of the dynamics of mesophilic and thermophilic dihydrofolate reductase is examined using elastic incoherent neutron scattering. It is demonstrated that the distribution of atomic displacement amplitudes can be derived from the elastic scattering data by assuming a (Weibull) functional form that resembles distributions seen in molecular dynamics simulations. The thermophilic enzyme has a significantly broader distribution than its mesophilic counterpart. Furthermore, although the rate of increase with temperature of the atomic mean-square displacements extracted from the dynamic structure factor is found to be comparable for both enzymes, the amplitudes are found to be slightly larger for the thermophilic enzyme. Therefore, these results imply that the thermophilic enzyme is the more flexible of the two.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherBiophysical Societyen_NZ
dc.relation.urihttp://www.biophysj.org/cgi/content/abstract/94/12/4812en_US
dc.rightsThis article has been published in the journal: Biophysical Journal. Copyright © 2008 by the Biophysical Society.en_US
dc.titleProtein dynamics and stability: The distribution of atomic fluctuations in thermophilic and mesophilic dihydrofolate reductase derived using elastic incoherent neutron scatteringen_US
dc.typeJournal Articleen_US
dc.identifier.doi10.1529/biophysj.107.121418en_NZ
dc.relation.isPartOfBiophysical Journalen_NZ
pubs.begin-page4812en_NZ
pubs.elements-id33134
pubs.end-page4818en_NZ
pubs.issue12en_NZ
pubs.volume94en_NZ


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