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dc.contributor.authorAndrews, Emma Sophie Vouten_NZ
dc.contributor.authorRzoska-Smith, Elizabethen_NZ
dc.contributor.authorArcus, Vickery L.en_NZ
dc.date.accessioned2020-09-10T21:14:33Z
dc.date.available2020-09-10T21:14:33Z
dc.date.issued2020en_NZ
dc.identifier.citationAndrews, E. S. V., Rzoska-Smith, E., & Arcus, V. L. (2020). Post-transcriptional modulation of the SigF regulon inMycobacterium smegmatisby the PhoH2 toxin-antitoxin. PLOS ONE, 15(7). https://doi.org/10.1371/journal.pone.0236551en
dc.identifier.issn1932-6203en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/13814
dc.description.abstractPhoH2 proteins are highly conserved across bacteria and archaea yet their biological function is poorly characterised. We examined the growth profiles of Mycobacterium smegmatis strains mc²155 and mc²155 ΔphoH2 and observed the same growth profile and growth rate in a variety of conditions. In light of the comparable growth, we used RNAseq to provide a snapshot of the differences between the transcriptomes of M. smegmatis mc²155 and M. smegmatis mc²155 ΔphoH2 during normal growth. At 48 hours, elevated expression of the sigF regulon was observed in ΔphoH2 relative to wild type. In biochemical assays, PhoH2 showed activity toward sigF mRNA insinuating a role of PhoH2 in modulating the pool of sigF mRNA in the cell during normal growth, adding further complexity to the repertoire of reported mechanisms of post-translational regulation. Multiple copies of the preferred target site of PhoH2 were identified in loops of the sigF mRNA structure, leading us to propose a mechanism for the activity of PhoH2 that is initiated after assembly on specific single-stranded loops of RNA. We hypothesise that PhoH2 is a toxin-antitoxin that contributes to the regulation of SigF at a post-transcriptional level through targeted activity on sigF mRNA. This work presents the first evidence for post-transcriptional regulation of SigF along with the biological function of PhoH2 from M. smegmatis. This has implications for the highly conserved PhoH2 toxin-antitoxin module across the mycobacteria including the important human pathogen M. tuberculosis.
dc.format.mimetypeapplication/pdf
dc.language.isoenen_NZ
dc.publisherPublic Library Scienceen_NZ
dc.rights© 2020 Andrews et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.subjectScience & Technologyen_NZ
dc.subjectMultidisciplinary Sciencesen_NZ
dc.subjectScience & Technology - Other Topicsen_NZ
dc.subjectMYCOBACTERIUM-SMEGMATISen_NZ
dc.subjectSTATIONARY-PHASEen_NZ
dc.subjectGENEen_NZ
dc.subjectEXPRESSIONen_NZ
dc.subjectPROTEINSen_NZ
dc.subjectHELICASEen_NZ
dc.subjectROLESen_NZ
dc.subjectHEATen_NZ
dc.titlePost-transcriptional modulation of the SigF regulon inMycobacterium smegmatisby the PhoH2 toxin-antitoxinen_NZ
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0236551en_NZ
dc.relation.isPartOfPLOS ONEen_NZ
pubs.elements-id255338
pubs.issue7en_NZ
pubs.publication-statusPublisheden_NZ
pubs.volume15en_NZ
uow.identifier.article-noARTN e0236551


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