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      Bacterial cellulose synthesis by gluconacetobacter xylinus: Enhancement via fed-batch fermentation strategies in Glycerol media

      Adnan, Azila; Nair, Giridhar; Lay, Mark C.; Swan, Janis E.
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      453-ArticleTextforProduction-791-1-10-20211111.pdf
      Published version, 339.1Kb
      DOI
       10.48048/tis.2021.453
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      Permanent link to Research Commons version
      https://hdl.handle.net/10289/15185
      Abstract
      Bacterial cellulose (BC) is an abundant polysaccharide, which is secreted by several genera of bacteria. It has remarkable characteristics, which include high purity, high tensile strength, high biocompatibility and non-toxic. The main feature that differentiates BC and plant cellulose (PC) is the absence of contaminants such as lignin, hemicellulose and pectin. However, the main drawbacks in producing BC are low yield and expensive carbon source. Due to that, this study was carried out to enhance BC volumetric productivity in fed-batch operation mode using glycerol as a carbon source. BC was produced in fill-and-draw and pulse-feed fed-batch cultures of Gluconacetobacter xylinus DSM 46604 in a 3-L bench-top bioreactor. The fed-batch fermentation trials were conducted in agitated and aerobic conditions at 30 ºC. For fill-and-draw fed-batch culture, a total of 24.2 g/L of BC accumulated in the bioreactor after 9 days, which corresponded to a yield and productivity of 0.2 g/g and 2.69 g/L/day, respectively. Pulse-feed fed-batch fermentation resulted in a yield and volumetric productivity of 0.38 g/g and 2.71 g/L/day, respectively. The pulse-feed fed-batch culture proved to be a better fermentation system for utilizing glycerol, which is a low-cost and abundant carbon source.
      Date
      2021
      Type
      Journal Article
      Rights
      This is an Open Access journal, by Walailak University.

      All material is licensed under a terms of the Creative Commons Attribution 4.0 International (CC-BY-NC-ND 4.0) licence, unless otherwise stated.
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      • Science and Engineering Papers [3122]
      • Health, Sport and Human Performance Papers [136]
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