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dc.contributor.authorWood, Susanna A.
dc.contributor.authorRhodes, Lesley L.
dc.contributor.authorAdams, Serean L.
dc.contributor.authorAdamson, Janet E.
dc.contributor.authorSmith, Kirsty Fiona
dc.contributor.authorSmith, John F.
dc.contributor.authorTervit, H. Robin
dc.contributor.authorCary, S. Craig
dc.date.accessioned2009-08-02T23:53:58Z
dc.date.available2009-08-02T23:53:58Z
dc.date.issued2008
dc.identifier.citationWood, S. A., Rhodes, L. L., Adams, S. L., Adamson, J. E., Smith, K. F., Smith, J. F., Tervit, H. R. & Cary, S. C. (2008). Maintenance of cyanotoxin production by cryopreserved cyanobacteria in the New Zealand culture collection. New Zealand Journal of Marine and Freshwater Research, 42(3), 277- 283.en
dc.identifier.urihttps://hdl.handle.net/10289/2726
dc.description.abstractA culture collection of freshwater planktonic and benthic cyanobacteria collected from sites across New Zealand has been established at the Cawthron Institute, Nelson, New Zealand. Limited resources led to uncertainty regarding the long-term maintenance of this collection. The present study demonstrates cryopreservation to be a viable method for long-term storage of cyanobacteria. Seventeen of 20 strains evaluated were successfully cryopreserved using the permeating cryopreservation agent dimethyl sulfoxide (Me2 SO), at a final concentration of 15% (v/v). Cyanotoxin analysis was undertaken on selected strains known to produce microcystins, nodularin, anatoxin-a, and saxitoxins. All strains retained their ability to produce these toxins following cryopreservation.en
dc.language.isoen
dc.publisherRSNZ Publishingen_NZ
dc.subjectdimethyl sulfoxideen
dc.subjectmicrocystinsen
dc.subjectnodularinen
dc.subjectanatoxin-aen
dc.subjectsaxitoxinsen
dc.titleMaintenance of cyanotoxin production by cryopreserved cyanobacteria in the New Zealand culture collectionen
dc.typeJournal Articleen
dc.identifier.doi10.1080/00288330809509955
dc.relation.isPartOfNew Zealand Journal of Marine and Freshwater Researchen_NZ
pubs.begin-page277en_NZ
pubs.elements-id33530
pubs.end-page283en_NZ
pubs.issue3en_NZ
pubs.volume42en_NZ


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