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      Cryosolvents useful for protein and enzyme studies below −100°C

      Reat, Valerie; Finney, John L.; Steer, Andrew; Roberts, Mark A.; Smith, Jeremy C.; Dunn, Rachel V.; Peterson, Michelle E.; Daniel, Roy M.
      DOI
       10.1016/S0165-022X(99)00053-6
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      Reat, V., Finney, J.L., Steer, A., Roberts, M.A., Smith, J., …, Daniel, R. M. (2000). Cryosolvents useful for protein and enzyme studies below −100°C. Journal of Biochemical and Biophysical Methods, 42(3), 97-103.
      Permanent Research Commons link: https://hdl.handle.net/10289/4461
      Abstract
      For the study of protein structure, dynamics, and function, at very low temperatures it is desirable to use cryosolvents that resist phase separation and crystallisation. We have examined these properties in a variety of cryosolvents. Using visual and X-ray diffraction criteria, methanol:ethanediol (70%:10%), methanol:glycerol (70%:10%), acetone:methoxyethanol:ethanediol (35%:35%:10%), dimethylformamide:ethanediol (70%:10%), dimethylformamide (80%), methoxyethanol (80%), and methoxyethanol:ethanediol (70%:10%) were all found to be free of phase-changes down to at least −160°C. The least viscous of these, methanol:ethanediol (70%:10%), was miscible down to −125°C and showed no exo or endothermic transitions when examined using DSC. It is therefore potentially particularly suitable for very low temperature cryoenzymology.
      Date
      2000
      Type
      Journal Article
      Publisher
      Elsevier
      Collections
      • Science and Engineering Papers [3121]
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