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      Assaying activity and assessing thermostability of hyperthermophilic enzymes

      Daniel, Roy M.; Danson, Michael J.
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      Daniel 2001 Assaying.pdf
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      DOI
       10.1016/S0076-6879(01)34476-2
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      Daniel, R.M. & Danson, M.J. (2001). Assaying activity and assessing thermostability of hyperthermophilic enzymes. Methods in Enzymology, 334, 283-293.
      Permanent Research Commons link: https://hdl.handle.net/10289/4472
      Abstract
      There is now a wide variety of intra- and extra-cellular enzymes available from organisms growing above 75°C, and having sufficient stability to allow assay well above this temperature. For some of these enzymes, to assay below even 95°C will involve measurement below the optimal growth temperature for the organism. The purpose of this chapter is to cover practical aspects of enzyme assay procedures that are specific to high temperatures. Since by far the commonest routine assessment of enzyme stability is activity loss, and because it is always unwise to measure enzyme activity without being confident of its stability during the assay, we include an outline of procedures for measuring enzyme activity loss/stability at high temperatures.
      Date
      2001
      Type
      Journal Article
      Publisher
      Elsevier
      Rights
      This is an author’s accepted version of an article published in the journal: Methods in Enzymology. Copyright © 2001 Published by Elsevier Inc.
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      • Science and Engineering Papers [3116]
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