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dc.contributor.authorRuttersmith, L.D.
dc.contributor.authorDaniel, Roy M.
dc.date.accessioned2010-08-31T22:09:28Z
dc.date.available2010-08-31T22:09:28Z
dc.date.issued1991
dc.identifier.citationRuttersmith, L.D. & Daniel, R.M. (1991). Thermostable cellobiohydrolase from the thermophilic eubacterium Thermotoga sp. strain FjSS3-B.1. Purification and properties. Biochemical Journal, 277(3), 887-890.en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/4494
dc.description.abstractExo-1,4-beta-cellobiohydrolase (EC 3.2.1.91) was isolated from the culture supernatant of Thermotoga sp. strain FjSS3-B.1, an extremely thermophilic eubacterium that grows optimally at 80 degrees C. The enzyme was purified to homogeneity as determined by SDS/PAGE and has an Mr of 36,000. The enzyme is the most thermostable cellulase reported to date, with a half-life at 108 degrees C of 70 min in buffer. In a 40 min assay, maximal activity was recorded at 105 degrees C. Cellobiohydrolase from strain FjSS3-B.1 is active against amorphous cellulose and CM-cellulose but only effects limited hydrolysis of filter paper or Sigmacell 20. The only product identified by h.p.l.c. is the disaccharide cellobiose. The enzyme has a pH optimum around neutral and is stabilized by the presence of 0.8 M-NaCl.en_NZ
dc.language.isoen
dc.relation.urihttp://www.biochemj.org/bj/277/bj2770887.htmen_NZ
dc.subjectbiologyen_NZ
dc.titleThermostable cellobiohydrolase from the thermophilic eubacterium Thermotoga sp. strain FjSS3-B.1. Purification and properties.en_NZ
dc.typeJournal Articleen_NZ


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