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      The cellulase activity of an extreme thermophile

      Hudson, J. Andrew; Morgan, Hugh W.; Daniel, Roy M.
      DOI
       10.1007/BF00184700
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      Hudson, J.A., Morgan, H.W. & Daniel, R.M. (1991). The cellulase activity of an extreme thermophile. Applied Microbiology and Biotechnology, 35(2), 270-273.
      Permanent Research Commons link: https://hdl.handle.net/10289/4498
      Abstract
      The carboxymethylcellulase activity concentrated from the extremely thermophilic anaerobe H173 was found to have a pH optimum of 6.5–7.0. The enzyme activity was stabilised by the addition of dithiothreitol and CaCl₂•2H₂O and was very stable at 80° C, retaining 77% of the initial activity after 120 min incubtation. At min and after 120 min only 3% of the initial activity remained. With the enzyme dissolved in buffer, glucose and cellobiose were formed from the hydrolosis of Avicel. In culture medium the Avicel-solubilising activity was insensitive to the presence of up tp 50 mm glucose and showed linear glucose accumulation over a period of days at 70° C. HPLC analysis established that glucose was the major end-product of hydrolysis in the culture broths.
      Date
      1991
      Type
      Journal Article
      Publisher
      Springer
      Collections
      • Science and Engineering Papers [3077]
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