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      Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome

      Health, Caroline; Hu, Xiao Ping; Cary, S. Craig; Cowan, Don A.
      DOI
       10.1128/AEM.02597-08
      Link
       aem.asm.org
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      Citation
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      Health, C., Hu, X.P., Cary, S.C. & Cowan, D. (2009). Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome. Applied and Environmental Microbiology, 77, 1153-1161.
      Permanent Research Commons link: https://hdl.handle.net/10289/5382
      Abstract
      A novel esterase was identified through functional screening of an Antarctic desert soil metagenomic library in E. coli. The 297-amino acid sequence had only low (< 29 %) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7-54 °C and at alkaline pH levels, and is a potential candidate for industrial application.
      Date
      2009
      Type
      Journal Article
      Publisher
      American Society for Microbiology
      Collections
      • Science and Engineering Papers [3073]
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