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dc.contributor.authorvan Haren, Frank MP
dc.contributor.authorSleigh, James W.
dc.contributor.authorCursons, Raymond T.
dc.contributor.authorPine, Mary L.
dc.contributor.authorPickkers, Peter
dc.contributor.authorvan der Hoeven, Johannes
dc.coverage.spatialGermanyen_NZ
dc.date.accessioned2011-11-18T03:16:34Z
dc.date.available2011-11-18T03:16:34Z
dc.date.issued2011
dc.identifier.citationvan Haren, F.M., Sleigh, J., Cursons, R., Pine, M.L., Pickkers, P. & van der Hoeven, J. (2011). The effects of hypertonic fluid administration on the gene expression of inflammatory mediators in circulating leucocytes in patients with septic shock: a preliminary study. Annals of Intensive Care, 1:44.en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/5904
dc.description.abstractObjective: This study was designed to investigate the effect of hypertonic fluid administration on inflammatory mediator gene expression in patients with septic shock. Design and setting: Prospective, randomized, controlled, double-blind clinical study in a 15-bed mixed intensive care unit in a tertiary referral teaching hospital. Interventions: Twenty-four patients, who met standard criteria for septic shock, were randomized to receive a bolus of hypertonic fluid (HT, 250 ml 6% HES/7.2% NaCl) or isotonic fluid (IT, 500 ml 6% HES/0.9% NaCl) administered over 15 minutes. Randomization and study fluid administration was within 24 hours of ICU admission for all patients. This trial is registered with ANZCTR.org.au as ACTRN12607000259448. Results: Blood samples were taken immediately before and 4, 8, 12, and 24 hours after fluid administration. Real-time reverse transcriptase polymerase chain reaction (RT rtPCR) was used to quantify mRNA expression of different inflammatory mediators in peripheral leukocytes. In the HT group, compared with the IT group, levels of gene expression of MMP9 and L-selectin were significantly suppressed (p = 0.0002 and p = 0.007, respectively), and CD11b gene expression tended to be elevated (p = NS). No differences were found in the other mediators examined. Conclusions: In septic shock patients, hypertonic fluid administration compared with isotonic fluid may modulate expression of genes that are implicated in leukocyte-endothelial interaction and capillary leakage. The study was performed at the Intensive Care Department, Waikato Hospital, and at the Molecular Genetics Laboratory, University of Waikato, Hamilton, New Zealand. Trial registration: Australia and New Zealand Clinical Trials Register (ANZCTR): ACTRN12607000259448en_NZ
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherSpringer Verlagen_NZ
dc.relation.urihttp://www.annalsofintensivecare.com/content/1/1/44en_NZ
dc.subjecthypertonic fluid administrationen_NZ
dc.subjectinflammatory mediatoren_NZ
dc.titleThe effects of hypertonic fluid administration on the gene expression of inflammatory mediators in circulating leucocytes in patients with septic shock: a preliminary studyen_NZ
dc.typeJournal Articleen_NZ
dc.identifier.doi10.1186/2110-5820-1-44en_NZ
dc.relation.isPartOfAnnals of Intensive Careen_NZ
pubs.begin-page1en_NZ
pubs.elements-id36686
pubs.end-page8en_NZ
pubs.issue44en_NZ
pubs.volume1en_NZ
uow.identifier.article-noARTN 44en_NZ


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