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      Decoloring hemoglobin as a feedstock for second-generation bioplastics

      Low, Aaron; Lay, Mark C.; Verbeek, Casparus Johan R.; Swan, Janis E.
      DOI
       10.1080/10826068.2011.563401
      Link
       www.tandfonline.com
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      Citation
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      Low, A., Lay, M.C., Verbeek, J. & Swan, J.E. (2012). Decoloring hemoglobin as a feedstock for second-generation bioplastics. Preparative Biochemistry and Biotechnology, 42(1), 29-43.
      Permanent Research Commons link: https://hdl.handle.net/10289/6351
      Abstract
      The color of red blood cell concentrate (RBCC) limits its application in human food, but there is potential to use it for second-generation bioplastics. Several methods have been developed to remove color from RBCC, but they are expensive or may produce difficult-to-remove toxic residues. Hydrogen peroxide treatment is a cheaper alternative. The effects of RBCC concentration, pH, and reaction temperature were the most important factors influencing the decolorizing process. They were investigated with the aim of developing a method that could be scaled to commercial level for producing a bioplastic feedstock. Initial trials showed pH was an important factor for decolorization and foaming. At pH 15 there was a 96% reduction in solution color and 8.4% solids were lost due to foaming. There was a 76% reduction in solution color at pH 2 and only 2.6% solids were lost due to foaming. The optimal reaction conditions were to centrifuge 9% w/w, pH 2 aqueous RBCC solution to remove aggregates. The solution was reacted at 30°C with 7.5 g of 30% (w/w) hydrogen peroxide. These conditions achieved a 93% reduction in solution color after 3 hr and the molecular weight of the decolored protein was not significantly reduced.
      Date
      2012
      Type
      Journal Article
      Publisher
      Taylor & Francis
      Collections
      • Science and Engineering Papers [3118]
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