Show simple item record  

dc.contributor.authorStacheter, Astrid
dc.contributor.authorNoll, Matthias
dc.contributor.authorLee, Charles Kai-Wu
dc.contributor.authorSelzer, Mirjam
dc.contributor.authorGlowik, Beate
dc.contributor.authorEbertsch, Linda
dc.contributor.authorMertel, Ralf
dc.contributor.authorSchulz, Daria
dc.contributor.authorLampert, Niclas
dc.contributor.authorDrake, Harold L.
dc.contributor.authorKolb, Steffen
dc.identifier.citationStacheter, A., Noll, M., Lee, C. K., Selzer, M., Glowik, B., Ebertsch, L., & Kolb, S. (2012). Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs. The ISME Journal advance publication online, December 2012.en_NZ
dc.description.abstractThe role of soil methylotrophs in methanol exchange with the atmosphere has been widely overlooked. Methanol can be derived from plant polymers and be consumed by soil microbial communities. In the current study, methanol-utilizing methylotrophs of 14 aerated soils were examined to resolve their comparative diversities and capacities to utilize ambient concentrations of methanol. Abundances of cultivable methylotrophs ranged from 10 ⁶-10 ⁸gsoilDW ⁻¹. Methanol dissimilation was measured based on conversion of supplemented 14C-methanol, and occurred at concentrations down to 0.002 μmol methanol gsoilDW ⁻¹. Tested soils exhibited specific affinities to methanol (a ⁰ s=0 ⁻¹) that were similar to those of other environments suggesting that methylotrophs with similar affinities were present. Two deep-branching alphaproteobacterial genotypes of mch responded to the addition of ambient concentrations of methanol (≤0.6 μmol methanol gsoilDW ⁻¹) in one of these soils. Methylotroph community structures were assessed by amplicon pyrosequencing of genes of mono carbon metabolism (mxaF, mch and fae). Alphaproteobacteria-affiliated genotypes were predominant in all investigated soils, and the occurrence of novel genotypes indicated a hitherto unveiled diversity of methylotrophs. Correlations between vegetation type, soil pH and methylotroph community structure suggested that plant-methylotroph interactions were determinative for soil methylotrophs.en_NZ
dc.publisherNature Publishing Groupen_NZ
dc.relation.ispartofThe ISME Journal
dc.titleMethanol oxidation by temperate soils and environmental determinants of associated methylotrophsen_NZ
dc.typeJournal Articleen_NZ
dc.relation.isPartOfThe ISME Journalen_NZ

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record