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      A sensitive genetic-based detection capability for Didymosphenia geminata

      Cary, S. Craig; Hicks, Brendan J.; Crawford, Naomi; Coyne, Kathryn J.
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      CBER_45.pdf
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      Link
       cber.bio.waikato.ac.nz
      Citation
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      Cary, S.C., Hicks, B.J., Crawford, N.J. & Coyne, K.J. (2006). A sensitive genetic-based detection capability for Didymosphenia geminata. CBER Contract Report No. 45, prepared for MAF Biosecurity New Zealand. Hamilton, New Zealand: Centre for Biodiversity and Ecology Research, The University of Waikato.
      Permanent Research Commons link: https://hdl.handle.net/10289/7214
      Abstract
      It is now well recognized that the increase in global transportation over the last two decades has brought with it an increased potential for the introduction of unwanted microorganisms (aquatic or terrestrial) that may have drastic effects on human and ecosystem health and agriculture. We have developed and validated a unique genetic fingerprinting tool for D. geminata. In concert, we developed field collection and preservation techniques specific for D. geminata along with genetic-based procedures that can now reliably detect D. geminate from a complex environmental community with a high degree of sensitivity. Recent work (Phase 2) has shown that the described methods will provide detection levels from <1 – 10,000 cells ml-1. We contend that the genetic based detection approaches used in this study offer great promise to meet the increasing demands to monitor the global threat from invasive micro-organisms.
      Date
      2006-12
      Type
      Commissioned Report for External Body
      Series
      CBER Contract Report
      Report No.
      No.45
      Publisher
      Centre for Biodiversity and Ecology Research, The University of Waikato
      Collections
      • Science and Engineering Papers [3077]
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