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dc.contributor.authorLi, Ronggai
dc.contributor.authorWang, Tiehui
dc.contributor.authorBird, Steve
dc.contributor.authorZou, Jun
dc.contributor.authorDooley, Helen
dc.contributor.authorSecombes, Christopher J.
dc.date.accessioned2013-04-17T04:45:36Z
dc.date.available2013-04-17T04:45:36Z
dc.date.copyright2013-02
dc.date.issued2013
dc.identifier.citationLi, R., Wang, T., Bird, S., Zou, J., Dooley, H., & Secombes, C. J. (2013). B cell receptor accessory molecule CD79α: Characterisation and expression analysis in a cartilaginous fish, the spiny dogfish (Squalus acanthias). Fish & Shellfish Immunology, 34(6), 1401-1415.en_NZ
dc.identifier.issn1050-4648
dc.identifier.urihttps://hdl.handle.net/10289/7470
dc.description.abstractCD79α (also known as Igα) is a component of the B cell antigen receptor complex and plays an important role in B cell signalling. The CD79α protein is present on the surface of B cells throughout their life cycle, and is absent on all other healthy cells, making it a highly reliable marker for B cells in mammals. In this study the spiny dogfish (Squalus acanthias) CD79α (SaCD79α) is described and its expression studied under constitutive and stimulated conditions. The spiny dogfish CD79α cDNA contains an open reading frame of 618 bp, encoding a protein of 205 amino acids. Comparison of the SaCD79α gene with that of other species shows that the gross structure (number of exons, exon/intron boundaries, etc.) is highly conserved across phylogeny. Additionally, analysis of the 5′ flanking region shows SaCD79α lacks a TATA box and possesses binding sites for multiple transcription factors implicated in its B cell-specific gene transcription in other species. Spiny dogfish CD79α is most highly expressed in immune tissues, such as spleen, epigonal and Leydig organ, and its transcript level significantly correlates with those of spiny dogfish immunoglobulin heavy chains. Additionally, CD79α transcription is up-regulated, to a small but significant degree, in peripheral blood cells following stimulation with pokeweed mitogen. These results strongly indicate that, as in mammals, spiny dogfish CD79α is expressed by shark B cells where it associates with surface-bound immunoglobulin to form a fully functional BCR, and thus may serve as a pan-B cell marker in future shark immunological studies.en_NZ
dc.language.isoen
dc.publisherElsevieren_NZ
dc.relation.ispartofFish & Shellfish Immunology
dc.subjectcartilaginous fishen_NZ
dc.subjectCD79αen_NZ
dc.subjectdogfishen_NZ
dc.subjectIgαen_NZ
dc.subjectImmunoglobulinen_NZ
dc.titleB cell receptor accessory molecule CD79α: Characterisation and expression analysis in a cartilaginous fish, the spiny dogfish (Squalus acanthias)en_NZ
dc.typeJournal Articleen_NZ
dc.identifier.doi10.1016/j.fsi.2013.02.015en_NZ
dc.relation.isPartOfFish & Shellfish Immunologyen_NZ
pubs.begin-page1404en_NZ
pubs.elements-id38855
pubs.end-page1415en_NZ
pubs.issue6en_NZ
pubs.volume34en_NZ


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