In vitro electrical conductivity of seizing and non-seizing mouse brain slices at 10 kHz
Elbohouty, M., Wilson, M. T., Voss, L. J., Steyn-Ross, D. A., & Hunt, L. A. (2013). In vitro electrical conductivity of seizing and non-seizing mouse brain slices at 10 kHz.. Physics in Medicine and Biology, 58(11), 3599-3613.
Permanent Research Commons link: https://hdl.handle.net/10289/7600
The electrical conductivity of small samples of mouse cortex (in vitro) has been measured at 10 kHz through the four-electrode method of van der Pauw. Brain slices from three mice were prepared under seizing and non-seizing conditions by changing the concentration of magnesium in the artificial cerebrospinal fluid used to maintain the tissue. These slices provided 121 square samples of cortical tissue; the conductivity of these samples was measured with an Agilent E4980A four-point impedance monitor. Of these, 73 samples were considered acceptable on the grounds of having good electrical contact between electrodes and tissue excluding outlier measurements. Results show that there is a significant difference (p = 0.03) in the conductivities of the samples under the two conditions. The seizing and non-seizing samples have mean conductivities of 0.33 and 0.36 S m⁻¹, respectively; however, these quantitative values should be used with caution as they are both subject to similar systematic uncertainties due to non-ideal temperature conditions and non-ideal placement of electrodes. We hypothesize that the difference between them, which is more robust to uncertainty, is due to the changing gap junction connectivity during seizures.
Institute of Physics