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dc.contributor.authorMiles, Christopher O.
dc.contributor.authorSandvik, Morten
dc.contributor.authorNonga, Hezron E.
dc.contributor.authorRundberget, Thomas
dc.contributor.authorWilkins, Alistair L.
dc.contributor.authorRise, Frode
dc.contributor.authorBallot, Andreas
dc.date.accessioned2013-06-07T04:52:22Z
dc.date.available2013-06-07T04:52:22Z
dc.date.copyright2013-08
dc.date.issued2013
dc.identifier.citationMiles, C. O., Sandvik, M., Nonga, H. E., Rundberget, T., Wilkins, A. L., Rise, F., & Ballot, A. (2013). Identification of microcystins in a Lake Victoria cyanobacterial bloom using LC–MS with thiol derivatization. Toxicon, 70, 21-31en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/7690
dc.description.abstractMicrocystins are cyclic heptapeptides from cyanobacteria which are responsible for poisonings of livestock and humans. Cyanobacteria also produce a range of peptides and other compounds that can result in complex chromatograms when samples are analysed by LC–MS. Thiol derivatization of the α,β-unsaturated amide present in most microcystins was recently shown to simplify analysis of LC–MS chromatograms of a Microcystis culture, making it easier to identify peaks corresponding to microcystins in complex mixtures. This method was applied to analysis of extracts taken from a natural cyanobacteria bloom in Mwanza Gulf, Lake Victoria, Tanzania, in 2010, revealing the presence of numerous putative microcystin analogues in the sample. Results were verified using LC–MS², LC–MS/MS with precursor-ion scanning, and LC–HRMS, leading to identification of 8 major and 17 minor microcystins in the sample, including analogues of microcystin-RY, -RL and -RA. Microcystin-YR (2), -RR (3), and -RY (9) were isolated from bloom material from Lake Victoria, and the structure of 9 was confirmed by NMR spectroscopic analysis and NMR spectral comparison with 2 and 3. Confirmation of the structure of MC-RY (9) facilitated detailed analysis of its MS² spectrum, thereby supporting the structures of related analogues tentatively established on the basis of MS analyses.en_NZ
dc.language.isoenen_NZ
dc.publisherElsevieren_NZ
dc.relation.ispartofToxicon
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S0041010113001116en_NZ
dc.subjectMicrocystinen_NZ
dc.subjectMicrocystisen_NZ
dc.subjectAfricaen_NZ
dc.subjectLake Victoriaen_NZ
dc.subjectLC–MSen_NZ
dc.subjectThiol derivatizationen_NZ
dc.titleIdentification of microcystins in a Lake Victoria cyanobacterial bloom using LC–MS with thiol derivatizationen_NZ
dc.typeJournal Articleen_NZ
dc.identifier.doi10.1016/j.toxicon.2013.03.016en_NZ


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