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dc.contributor.authorMcFarlane, Craig Desmond
dc.contributor.authorHennebry, Alex
dc.contributor.authorThomas, Mark
dc.contributor.authorPlummer, Erin
dc.contributor.authorLing, Nicholas
dc.contributor.authorSharma, Mridula
dc.contributor.authorKambadur, Ravi
dc.coverage.spatialUnited Statesen_NZ
dc.date.accessioned2013-11-22T01:24:47Z
dc.date.available2013-11-22T01:24:47Z
dc.date.copyright2008-01
dc.date.issued2008
dc.identifier.citationMcFarlane, C., Hennebry, A., Thomas, M., Plummer, E., Ling, N., Sharma, M., & Kambadur, R. (2008). Myostatin signals through Pax7 to regulate satellite cell self-renewal. Experimental Cell Research, 314(2), 317-329.en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/8219
dc.description.abstractMyostatin, a Transforming Growth Factor-beta (TGF-β) super-family member, has previously been shown to negatively regulate satellite cell activation and self-renewal. However, to date the mechanism behind Myostatin function in satellite cell biology is not known. Here we show that Myostatin signals via a Pax7-dependent mechanism to regulate satellite cell self-renewal. While excess Myostatin inhibited Pax7 expression via ERK1/2 signaling, an increase in Pax7 expression was observed following both genetic inactivation and functional antagonism of Myostatin. As a result, we show that either blocking or inactivating Myostatin enhances the partitioning of the fusion-incompetent self-renewed satellite cell lineage (high Pax7 expression, low MyoD expression) from the pool of actively proliferating myogenic precursor cells. Consistent with this result, over-expression of Pax7 in C2C12 myogenic cells resulted in increased self-renewal through a mechanism which slowed both myogenic proliferation and differentiation. Taken together, these results suggest that increased expression of Pax7 promotes satellite cell self-renewal, and furthermore Myostatin may control the process of satellite cell self-renewal through regulation of Pax7. Thus we speculate that, in addition to the intrinsic factors (such as Pax7), extrinsic factors both positive and negative in nature, will play a major role in determining the stemness of skeletal muscle satellite cells.en_NZ
dc.language.isoenen_NZ
dc.publisherAcademic Pressen_NZ
dc.relation.ispartofExperimental Cell Research
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S0014482707004430en_NZ
dc.subjectMyostatinen_NZ
dc.subjectPax7en_NZ
dc.subjectMyoDen_NZ
dc.subjectsatellite cellen_NZ
dc.subjectself-renewalen_NZ
dc.subjecterk1/2en_NZ
dc.subjectreserve cellen_NZ
dc.titleMyostatin signals through Pax7 to regulate satellite cell self-renewalen_NZ
dc.typeJournal Articleen_NZ
dc.identifier.doi10.1016/j.yexcr.2007.09.012en_NZ
dc.relation.isPartOfExperimental Cell Researchen_NZ
pubs.begin-page317en_NZ
pubs.elements-id32930
pubs.end-page329en_NZ
pubs.issue2en_NZ
pubs.volume314en_NZ


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