The orphan G protein-coupled receptor gene GPR178 is evolutionary conserved and altered in response to acute changes in food intake

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dc.contributor.authorCaruso, Vannien_NZ
dc.contributor.authorLe Grevés, Madeleineen_NZ
dc.contributor.authorFard, Shahrzad Shirazien_NZ
dc.contributor.authorHaitina, Tatjanaen_NZ
dc.contributor.authorOlszewski, Pawel K.en_NZ
dc.contributor.authorAlsiö, Johanen_NZ
dc.contributor.authorSchiöth, Helgi B.en_NZ
dc.contributor.authorFredriksson, Roberten_NZ
dc.date.accessioned2015-09-02T21:49:12Z
dc.date.available2015-06-05en_NZ
dc.date.available2015-09-02T21:49:12Z
dc.date.issued2015-06-05en_NZ
dc.description.abstractG protein-coupled receptors (GPCRs) are a class of integral membrane proteins mediating physiological functions fundamental for survival, including energy homeostasis. A few years ago, an amino acid sequence of a novel GPCR gene was identified and named GPR178. In this study, we provide new insights regarding the biological significance of Gpr178 protein, investigating its evolutionary history and tissue distribution as well as examining the relationship between its expression level and feeding status. Our phylogenetic analysis indicated that GPR178 is highly conserved among all animal species investigated, and that GPR178 is not a member of a protein family. Real-time PCR and in situ hybridization revealed wide expression of Gpr178 mRNA in both the brain and periphery, with high expression density in the hypothalamus and brainstem, areas involved in the regulation of food intake. Hence, changes in receptor expression were assessed following several feeding paradigms including starvation and overfeeding. Short-term starvation (12-48h) or food restriction resulted in upregulation of Gpr178 mRNA expression in the brainstem, hypothalamus and prefrontal cortex. Conversely, short-term (48h) exposure to sucrose or Intralipid solutions downregulated Gpr178 mRNA in the brainstem; long-term exposure (10 days) to a palatable high-fat and high-sugar diet resulted in a downregulation of Gpr178 in the amygdala but not in the hypothalamus. Our results indicate that hypothalamic Gpr178 gene expression is altered during acute exposure to starvation or acute exposure to palatable food. Changes in gene expression following palatable diet consumption suggest a possible involvement of Gpr178 in the complex mechanisms of feeding reward.en_NZ
dc.format.mimetypeapplication/pdf
dc.identifier.citationCaruso, V., Le Grevés, M., Fard, S. S., Haitina, T., Olszewski, P. . ., Alsiö, J., … Fredriksson, R. (2015). The orphan G protein-coupled receptor gene GPR178 is evolutionary conserved and altered in response to acute changes in food intake. PLoS ONE, 10(6). http://doi.org/10.1371/journal.pone.0122061en
dc.identifier.doi10.1371/journal.pone.0122061en_NZ
dc.identifier.eissn1932-6203en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/9588
dc.language.isoen
dc.publisherPublic Library of Scienceen_NZ
dc.relation.isPartOfPLoS ONEen_NZ
dc.relation.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0122061en_NZ
dc.rights© 2015 Caruso et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.titleThe orphan G protein-coupled receptor gene GPR178 is evolutionary conserved and altered in response to acute changes in food intakeen_NZ
dc.typeJournal Article
pubs.begin-pagee0122061en_NZ
pubs.elements-id121507
pubs.end-pagee0122061en_NZ
pubs.issue6en_NZ
pubs.notesEvidence of peer review: http://journals.plos.org/plosone/s/journal-informationen_NZ
pubs.organisational-group/Waikato
pubs.organisational-group/Waikato/FSEN
pubs.organisational-group/Waikato/FSEN/School of Science
pubs.volume10en_NZ
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