Investigating diet as the source of tetrodotoxin in Pleurobranchaea maculata

Simple item page
dc.contributor.authorKhor, Serena
dc.contributor.authorWood, Susanna A.
dc.contributor.authorSalvitti, Lauren R.
dc.contributor.authorTaylor, David I.
dc.contributor.authorAdamson, Janet E.
dc.contributor.authorMcNabb, Paul
dc.contributor.authorCary, S. Craig
dc.coverage.spatialSwitzerlanden_NZ
dc.date.accessioned2014-12-14T22:42:12Z
dc.date.available2014-01
dc.date.available2014-12-14T22:42:12Z
dc.date.issued2014-01
dc.description.abstractThe origin of tetrodotoxin (TTX) is highly debated; researchers have postulated either an endogenous or exogenous source with the host accumulating TTX symbiotically or via food chain transmission. The aim of this study was to determine whether the grey side-gilled sea slug (Pleurobranchaea maculata) could obtain TTX from a dietary source, and to attempt to identify this source through environmental surveys. Eighteen non-toxic P. maculata were maintained in aquariums and twelve were fed a TTX-containing diet. Three P. maculata were harvested after 1 h, 24 h, 17 days and 39 days and TTX concentrations in their stomach, gonad, mantle and remaining tissue/fluids determined using liquid chromatography-mass spectrometry. Tetrodotoxin was detected in all organs/tissue after 1 h with an average uptake of 32%. This decreased throughout the experiment (21%, 15% and 9%, respectively). Benthic surveys at sites with dense populations of toxic P. maculata detected very low or no TTX in other organisms. This study demonstrates that P. maculata can accumulate TTX through their diet. However, based on the absence of an identifiable TTX source in the environment, in concert with the extremely high TTX concentrations and short life spans of P. maculata, it is unlikely to be the sole TTX source for this species.
dc.format.extent1 - 16
dc.format.mimetypeapplication/pdf
dc.identifier.citationKhor, S., Wood, S. A., Salvitti, L. R., Taylor, D. I., Adamson, J. E., McNabb, P., & Cary, S. C. (2014). Investigating diet as the source of tetrodotoxin in Pleurobranchaea maculata. Marine Drugs, 12(1), 1–16. http://doi.org/10.3390/md12010001en
dc.identifier.doi10.3390/md12010001
dc.identifier.eissn1660-3397
dc.identifier.urihttps://hdl.handle.net/10289/8966
dc.languageeng
dc.language.isoen
dc.publisherMolecular Dive Prsyn Intl
dc.relation.isPartOfMarine Drugs
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License.
dc.titleInvestigating diet as the source of tetrodotoxin in Pleurobranchaea maculata
dc.typeJournal Article
pubs.begin-page1
pubs.elements-id83772
pubs.end-page16
pubs.issue1
pubs.notesEBSCOhost confirms peer reviewed
pubs.organisational-group/Waikato
pubs.organisational-group/Waikato/FSEN
pubs.organisational-group/Waikato/FSEN/School of Science
pubs.organisational-group/Waikato/FSEN/School of Science/Biological Sciences
pubs.volume12
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2014 khor wood salvitti taylor adamson mcnab cary Marine Drugs.pdf
Size:
792.43 KB
Format:
Adobe Portable Document Format
Description:
Published version
Thumbnail Image
Name: 2014 khor wood salvitti taylor adamson mcnab cary Marine Drugs.pdf
Size: 792.43 KB
Kind: Adobe PDF
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Deposit Agreement.txt
Size:
193 B
Format:
Unknown data format
Description:
Name: Deposit Agreement.txt
Size: 193 B
Kind: Unknown
Collections
Science and Engineering Papers