Identifying diazotrophs by incorporation of nitrogen from ¹⁵N₂ into RNA

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dc.contributor.authorAddison, Sarah Louise
dc.contributor.authorMcDonald, Ian R.
dc.contributor.authorLloyd-Jones, Gareth
dc.coverage.spatialGermanyen_NZ
dc.date.accessioned2010-08-08T23:48:50Z
dc.date.available2010-08-08T23:48:50Z
dc.date.issued2010
dc.description.abstractThe diversity and abundance of active diazotrophs was investigated in a New Zealand pulp and paper wastewater by enrichment with ¹⁵N₂. Purified ¹⁵N-RNA was analysed by reverse transcription, molecular cloning and sequence analysis of 16S rRNA to reveal a diverse community of bacteria as indicated by a Shannon Weaver Index value of > 2.8. The major class represented in the enriched culture were the γ-Proteobacteria at 85% with a secondary group of the phylum Firmicutes present at 8.2%, the remaining sequences were affiliated with the α- and β-Proteobacterial classes (1.4% and 4.3%, respectively). Three dominant genera, Aeromonas, Pseudomonas and Bacillus, were identified by comparison with published sequences and phylogenetic analysis. To confirm that representatives of the taxonomic groups identified from the active enriched nitrogen-fixing community were capable of fixing nitrogen Aeromonas and Pseudomonas species were cultivated and shown to possess nifH genes. In wastewater, fluorescence in situ hybridisation probing revealed that the dominant nitrogen-fixing population identified in this study were present in the population, but at lower levels. The population is, therefore, reliant on a small sub-population of diazotrophs to supply the community's nitrogen needs above that already present in the wastewater.en_NZ
dc.identifier.citationAddison, S.L., McDonald, I.R. & Lloyd-Jones, G. (2010). Identifying diazotrophs by incorporation of nitrogen from ¹⁵N₂ into RNA. Applied Microbiology and Biotechnology, 87(6), 2313-2322.en_NZ
dc.identifier.doi10.1007/s00253-010-2731-zen_NZ
dc.identifier.urihttps://hdl.handle.net/10289/4261
dc.language.isoen
dc.publisherSpringeren_NZ
dc.relation.isPartOfApplied microbiology and biotechnologyen_NZ
dc.relation.urihttp://www.springerlink.com/content/d400k743038rr83t/en_NZ
dc.subjectnitrogen fixingen_NZ
dc.subjectstable isotope probing (SIP)en_NZ
dc.subjectwastewateren_NZ
dc.subjectPseudomonasen_NZ
dc.subject¹⁵N₂-labellingen_NZ
dc.titleIdentifying diazotrophs by incorporation of nitrogen from ¹⁵N₂ into RNAen_NZ
dc.typeJournal Articleen_NZ
pubs.begin-page2313en_NZ
pubs.elements-id35204
pubs.end-page2322en_NZ
pubs.issue6en_NZ
pubs.volume87en_NZ
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