The decrease in mature myostatin protein in male skeletal muscle is developmentally regulated by growth hormone

Simple item page
dc.contributor.authorOldham, Jenny M.
dc.contributor.authorOsepchook, Claire
dc.contributor.authorJeanplong, Ferenc
dc.contributor.authorFalconer, Shelley J.
dc.contributor.authorMatthews, Kenneth
dc.contributor.authorConaglen, John V.
dc.contributor.authorGerrard, David F.
dc.contributor.authorSmith, Heather K.
dc.contributor.authorWilkins, Richard J.
dc.contributor.authorBass, James J.
dc.contributor.authorMcMahon, Christopher D.
dc.date.accessioned2010-06-17T05:05:29Z
dc.date.available2010-06-17T05:05:29Z
dc.date.issued2009
dc.description.abstractMyostatin inhibits myogenesis and there is reduced abundance of the mature protein in skeletal muscles of adult male compared with female mice. This reduction probably occurs after translation, which suggests that it is a regulated mechanism to reduce the availability of myostatin in males. Reduced myostatin may, thereby, contribute to the development of sexually dimorphic growth of skeletal muscle. Our first objective was to determine if the decrease in mature myostatin protein occurs before the linear growth phase to aid growth, or afterwards to maintain the mass of adult muscle. Mice were killed from 2 to 32 weeks and the gastrocnemius muscle was excised. Myostatin mRNA increased from 2 to 32 weeks and was higher in males than females (P < 0.001). In contrast, mature protein decreased in males after 6 weeks (P < 0.001). Our second objective was to determine if growth hormone (GH) induces the decrease in mature myostatin protein. GH increased myostatin mRNA and decreased the abundance of mature protein in hypophysectomised mice (P < 0.05). Our final objective was to determine if the decrease in mature protein occurs in skeletal muscles of male Stat5b−/− mice (Stat5b mediates the actions of GH). As expected, mature myostatin protein was not reduced in Stat5b−/− males compared with females. However, myostatin mRNA remained higher in males than females irrespective of genotype. These data suggest that: (1) the decrease in mature myostatin protein is developmentally regulated, (2) GH acting via Stat5b regulates the abundance of mature myostatin and (3) GH acts via a non-Stat5b pathway to regulate myostatin mRNA.en_NZ
dc.identifier.citationOldham, J.M., Osepchook, C.C., Jeanplong, F., Falconer, S.J., Matthews, K.G.,…, McMahon, C.D. (2009). The decrease in mature myostatin protein in male skeletal muscle is developmentally regulated by growth hormone. The Journal of Physiology, 587(3), 669-677.en_NZ
dc.identifier.doi10.1113/jphysiol.2008.161521en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/4011
dc.language.isoen
dc.subjectmyostatinen_NZ
dc.subjectmuscleen_NZ
dc.subjectskeletal muscleen_NZ
dc.titleThe decrease in mature myostatin protein in male skeletal muscle is developmentally regulated by growth hormoneen_NZ
dc.typeJournal Articleen_NZ
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Name: license.txt
Size: 1.71 KB
Kind: License
Collections
Science and Engineering Papers