High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils

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dc.contributor.authorZablocki, Oliveren_NZ
dc.contributor.authorvan Zyl, Lonnieen_NZ
dc.contributor.authorAdriaenssens, Evelien M.en_NZ
dc.contributor.authorRubagotti, Enricoen_NZ
dc.contributor.authorTuffin, Marla I.en_NZ
dc.contributor.authorCary, S. Craigen_NZ
dc.contributor.authorCowan, Don A.en_NZ
dc.date.accessioned2015-09-03T04:35:40Z
dc.date.available2014-11-01en_NZ
dc.date.available2015-09-03T04:35:40Z
dc.date.issued2014-11-01en_NZ
dc.description.abstractThe metaviromes of two distinct Antarctic hyperarid desert soil communities have been characterized. Hypolithic communities, cyanobacterium-dominated assemblages situated on the ventral surfaces of quartz pebbles embedded in the desert pavement, showed higher virus diversity than surface soils, which correlated with previous bacterial community studies. Prokaryotic viruses (i.e., phages) represented the largest viral component (particularly Mycobacterium phages) in both habitats, with an identical hierarchical sequence abundance of families of tailed phages (Siphoviridae > Myoviridae > Podoviridae). No archaeal viruses were found. Unexpectedly, cyanophages were poorly represented in both metaviromes and were phylogenetically distant from currently characterized cyanophages. Putative phage genomes were assembled and showed a high level of unaffiliated genes, mostly from hypolithic viruses. Moreover, unusual gene arrangements in which eukaryotic and prokaryotic virus-derived genes were found within identical genome segments were observed. Phycodnaviridae and Mimiviridae viruses were the second-most-abundant taxa and more numerous within open soil. Novel virophage-like sequences (within the Sputnik clade) were identified. These findings highlight high-level virus diversity and novel species discovery potential within Antarctic hyperarid soils and may serve as a starting point for future studies targeting specific viral groups.en_NZ
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dc.identifier.citationZablocki, O., van Zyl, L., Adriaenssens, E. M., Rubagotti, E., Tuffin, M. I., Cary, S. C., & Cowan, D. A. (2014). High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils. Applied and Environmental Microbiology, 80(22), 6888–6897. http://doi.org/10.1128/AEM.01525-14en
dc.identifier.doi10.1128/AEM.01525-14en_NZ
dc.identifier.eissn1098-5336en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/9592
dc.language.isoen
dc.publisherAmerican Society Microbiologyen_NZ
dc.relation.isPartOfApplied and Environmental Microbiologyen_NZ
dc.relation.urihttp://aem.asm.org/content/80/22/6888en_NZ
dc.rights© 2014 American Society for Microbiology. Used with permission.
dc.titleHigh-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soilsen_NZ
dc.typeJournal Article
pubs.begin-page6888
pubs.elements-id120774
pubs.end-page6897
pubs.issue22en_NZ
pubs.notesEBSCOhost confirms peer revieweden_NZ
pubs.organisational-group/Waikato
pubs.organisational-group/Waikato/FSEN
pubs.organisational-group/Waikato/FSEN/School of Science
pubs.volume80en_NZ
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