Flow cytometric characterization of freshwater crayfish hemocytes for the examination of physiological status in wild and captive animals

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dc.contributor.authorTaylor, Sean
dc.contributor.authorLandman, Michael J.
dc.contributor.authorLing, Nicholas
dc.coverage.spatialUnited Statesen_NZ
dc.date.accessioned2009-12-06T20:06:54Z
dc.date.available2009-12-06T20:06:54Z
dc.date.issued2009
dc.description.abstractEnumeration of invertebrate hemocytes is a potentially powerful tool for the determination of physiological effects of extrinsic stressors, such as hypoxia, disease, and toxicant exposure. A detailed flow cytometric method of broad application was developed for the objective characterization and enumeration of the hemocytes of New Zealand freshwater crayfish Paranephrops planifrons for the purpose of physiological health assessment. Hemocyte populations were isolated by flow cytometric sorting based on differential light scatter properties followed by morphological characterization via light microscopy and software image analysis. Cells were identified as hyaline, semigranular, and granular hemocytes based on established invertebrate hemocyte classification. A characteristic decrease in nuclear size, an increase in granularity between the hyaline and granular cells, and the eccentric location of nuclei in granular cells were also observed. The granulocyte subpopulations were observed to possess varying degrees of granularity. The developed methodology was used to perform total and differential hemocyte counts from three lake populations and between wild and captive crayfish specimens. Differences in total and differential hemocyte counts were not observed among the wild populations. However, specimens held in captivity for 14 d exhibited a significant 63% reduction in total hemocyte count, whereas the relative hemocyte proportions remained the same. These results demonstrate the utility of this method for the investigation of subacute stressor effects in selected decapod crustaceans.en
dc.format.mimetypeapplication/pdf
dc.identifier.citationTaylor, S., Landman, M. J., & Ling, N. (2009). Flow cytometric characterization of freshwater crayfish hemocytes for the examination of physiological status in wild and captive animals. Journal of Aquatic Animal Health, 21(3), 195-203.en
dc.identifier.doi10.1577/H09-003.1en
dc.identifier.urihttps://hdl.handle.net/10289/3468
dc.language.isoen
dc.publisherAmerican Fisheries Societyen_NZ
dc.relation.isPartOfJournal of Aquatic Animal Healthen_NZ
dc.subjectNew Zealanden
dc.subjectcrayfishen
dc.subjectinvertebrateen
dc.subjecthemocyteen
dc.titleFlow cytometric characterization of freshwater crayfish hemocytes for the examination of physiological status in wild and captive animalsen
dc.typeJournal Articleen
pubs.begin-page195en_NZ
pubs.elements-id34510
pubs.end-page203en_NZ
pubs.issue3en_NZ
pubs.volume21en_NZ
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