Research Commons
      • Browse 
        • Communities & Collections
        • Titles
        • Authors
        • By Issue Date
        • Subjects
        • Types
        • Series
      • Help 
        • About
        • Collection Policy
        • OA Mandate Guidelines
        • Guidelines FAQ
        • Contact Us
      • My Account 
        • Sign In
        • Register
      View Item 
      •   Research Commons
      • University of Waikato Theses
      • Higher Degree Theses
      • View Item
      •   Research Commons
      • University of Waikato Theses
      • Higher Degree Theses
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      A Comparative Analysis of Equine Mesenchymal Stromal Cells and Dermal Fibroblasts

      Leander, Alexandra Anneli Kristina
      Thumbnail
      Files
      thesis.pdf
      4.939Mb
      Citation
      Export citation
      Leander, A. A. K. (2017). A Comparative Analysis of Equine Mesenchymal Stromal Cells and Dermal Fibroblasts (Thesis, Master of Philosophy (MPhil)). The University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/11718
      Permanent Research Commons link: https://hdl.handle.net/10289/11718
      Abstract
      The aim of this study was to isolate and characterise equine mesenchymal stromal cells from various sources and to compare their biological properties to that of dermal fibroblasts. By using multiple conventional methods a comparative analysis of the different populations could be made. The results showed that little distinguishes mesenchymal stromal cells from dermal fibroblasts, they have the same morphology and growth characteristics, the same cell surface markers as demonstrated with immunocytochemistry and flow cytometry and the capacity to undergo trilineage differentiation into adipo-, chondro- and osteogenic lineages essentially becoming fat, cartilage and bone. An allogenic synovial fluid model was shown to induce a chondrogenic phenotype in equine mesenchymal stromal cells as well as dermal fibroblasts. A bio-activation assay was performed to evaluate cytokine production of cell populations after activation with Tumour necrosis factor alpha or inflammatory synovial fluid. All of the analysed cell populations demonstrated up-regulation of production of the anti-inflammatory cytokines IL-10 and TGF-β1, after activation with inflammatory synovial fluid, although significant values could not be obtained for every source. As for Prostaglandin E2, results were obtained for bone marrow stromal cells and dermal fibroblasts populations only. Their effects were in opposite; dermal fibroblast reduced the production of Prostaglandin E2 post activation. A functional bio-activation assay has shown promise as a method to distinguish equine mesenchymal stromal cells from dermal fibroblast populations.
      Date
      2017
      Type
      Thesis
      Degree Name
      Master of Philosophy (MPhil)
      Supervisors
      Cursons, Raymond T.
      Morris, Lee
      Peters, Linda M.
      Publisher
      The University of Waikato
      Rights
      All items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
      Collections
      • Higher Degree Theses [1714]
      Show full item record  

      Usage

      Downloads, last 12 months
      190
       
       

      Usage Statistics

      For this itemFor all of Research Commons

      The University of Waikato - Te Whare Wānanga o WaikatoFeedback and RequestsCopyright and Legal Statement