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dc.contributor.authorMills, Heidi Clare Mariaen_NZ
dc.date.accessioned2007-12-19T11:33:42Z
dc.date.available2008-05-02T16:04:08Z
dc.date.issued2007en_NZ
dc.identifier.citationMills, H. C. M. (2007). Development of a Method of Analysis by High Performance Liquid Chromatography for Products of the Nitric Acid Oxidation of D-Glucose (Thesis, Master of Engineering (ME)). The University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/2517en
dc.identifier.urihttps://hdl.handle.net/10289/2517
dc.description.abstractThis thesis explored the development of a faster and more efficient means of qualitative and quantitative analysis of the products of the nitric acid oxidation of D-glucose and other simple sugars, for the Shafizadeh Rocky Mountain Center for Wood and Carbohydrate Chemistry. During the research, analysis was carried out based on previous work completed in a similar area using two Aminex HPX-87H+ cation-exchange columns at different temperatures, and plumbed in series. Standards were filtered and injected on to the columns, then eluted with 5 mM sulfuric acid. A total run time of 33 minutes enabled the elution of all products and by-products of the reaction. Retention times of standards and the use of spiking helped specify and quantify unknowns in samples from a series of oxidation reactions involving D-glucose and other aldoses. The PrevailTM Organic acid (OA) column was said to provide 'unsurpassed resolution of organic acids'. It was therefore investigated, and a method was developed and refined in order to optimise conditions enabling the column's use for the required analyses. The optimised parameters were established as: ambient temperature with an eluent of 10 mM KH2PO4 adjusted to a pH of 2.1 with phosphoric acid. The sample size was 5 uL with a flow rate of 0.3 mL/min, giving a total run time of approximately 13 minutes. The Aminex HPX-87H+ column method and the PrevailTM OA method were compared to determine the superior method for the analyses intended. While some improvements were made for detection in the PrevailTM OA method, results were not satisfactory. This was due in part to limits imposed on the PrevailTM OA column method, which prevented the use of gradient elution. The Aminex HPX-87H+ column method outlined herein provides superior resolution for the nitric acid oxidation of D-glucose to D-glucaric acid, and in conclusion it is suggested that the Aminex HPX-87H+ column method be used.en_NZ
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherThe University of Waikatoen_NZ
dc.rightsAll items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectAminex HPX-87Hen_NZ
dc.subjectPrevail OAen_NZ
dc.subjectchromatographyen_NZ
dc.subjectHPLCen_NZ
dc.subjectnitric acid oxidationen_NZ
dc.subjectD-glucoseen_NZ
dc.subjectorganic acidsen_NZ
dc.titleDevelopment of a Method of Analysis by High Performance Liquid Chromatography for Products of the Nitric Acid Oxidation of D-Glucoseen_NZ
dc.typeThesisen_NZ
thesis.degree.disciplineChemistryen_NZ
thesis.degree.grantorUniversity of Waikatoen_NZ
thesis.degree.levelMasters
thesis.degree.nameMaster of Engineering (ME)en_NZ
uow.date.accession2007-12-19T11:33:42Zen_NZ
uow.date.available2008-05-02T16:04:08Zen_NZ
uow.identifier.adthttp://adt.waikato.ac.nz/public/adt-uow20071219.113342en_NZ
uow.date.migrated2009-06-09T23:30:48Zen_NZ
pubs.place-of-publicationHamilton, New Zealanden_NZ


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