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dc.contributor.authorBoult, Cherie Helenen_NZ
dc.date.accessioned2010-02-16T20:41:32Z
dc.date.available2010-02-16T20:41:32Z
dc.date.issued2009en_NZ
dc.identifier.citationBoult, C. H. (2009). Development of a Method of Analysis by Gas Chromatography-Mass Spectrometry using Selected Ion Monitoring for the Measurement of Fish Sex Steroids in Water (Thesis). The University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/3581en
dc.identifier.urihttps://hdl.handle.net/10289/3581
dc.description.abstractA method for the analysis of testosterone, androstenedione, 5β-dihydrotestosterone, 11-ketotestosterone, estrone, 17β-estradiol, 17α-hydroxyprogesterone, 17α,20β-dihydroxy-4-pregnen-3-one, 5β-pregnane-3α,17α,20α-triol, 5β-androstane-3α,17β-diol, 5β-androstane-3β,17β-diol, 5β-pregnane-3α,17α,20β-triol, 5β-androstane-3α,11β-diol-17-one, 5β-pregnane-3α,17α-diol-20-one, 17α,20β,21-trihydroxy-pregn-4-en-3-one and 11-deoxycortisol in water was developed using gas chromatography-mass spectrometry with selected ion monitoring. Fish sex steroids were extracted from holding water using C18 SPE cartridges, and eluted using methanol. Free and glucuronide conjugate levels of steroids were determined, glucuronide conjugates by hydrolysis using β-glucuronidase prior to derivatisation and free steroids by direct derivatisation. The method thus developed was applied to twelve goldfish holding water samples. Androstenedione was found to be excreted in considerably higher quantities by female ovulatory goldfish than spermiating goldfish, in contrast to previous research in this area.en_NZ
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherThe University of Waikatoen_NZ
dc.rightsAll items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectSex Steroidsen_NZ
dc.subjectGas Chromatography-Mass Spectrometryen_NZ
dc.subjectSolid Phase Extractionen_NZ
dc.titleDevelopment of a Method of Analysis by Gas Chromatography-Mass Spectrometry using Selected Ion Monitoring for the Measurement of Fish Sex Steroids in Wateren_NZ
dc.typeThesisen_NZ
thesis.degree.disciplineChemistryen_NZ
thesis.degree.grantorUniversity of Waikatoen_NZ
thesis.degree.levelMasters
uow.date.accession2009-06-23T14:23:42Zen_NZ
uow.identifier.adthttp://adt.waikato.ac.nz/public/adt-uow20090623.142342
pubs.place-of-publicationHamilton, New Zealanden_NZ


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