Removal of flatulence causing sugars in soymilk
Rajan, A. (2009). Removal of flatulence causing sugars in soymilk (Thesis, Master of Engineering (ME)). The University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/3604
Permanent Research Commons link: https://hdl.handle.net/10289/3604
The enzyme, alpha-galactosidase was extracted from peanuts using various buffersand solvents. The specific activity of soluble preparation of peanut ranged from0.0083 to 0.0606 U/mg. Phosphate buffer extract showed the maximum specificactivity of 0.0606 U/mg. The soluble alpha-galactosidase was immobilised in calciumalginate beads and the specific activity of the immobilized enzyme was found tobe 0.0808 U/mg. Activity yield was 33%. The immobilised enzyme showedincrease in activity compared with the soluble enzyme above 35 C. The thermalstability of the immobilised alpha-galactosidase was significantly improved incomparison to the soluble form. The effect of different pH depicts that at acidicpH the activity of the immobilised alpha-galactosidase was higher than that of thesoluble enzyme. The Km and Vmax for immobilised enzyme was higher whencompared with the soluble enzyme. Immobilised alpha-galactosidase retained 20%activity after 7 repeated uses. The immobilised enzyme exhibited high storagestability. The immobilised enzyme was used in batch and continuous packed bedreactors for the hydrolysis of stachyose and raffinose in soymilk. Flatulencecausing raffinose and stachyose, after hydrolysis, was removed to a remarkableextent. Hydrolysis of flatulence causing oligosaccharides of soymilk in acontinuous packed bed reactor has not been attempted previously.
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- Masters Degree Theses