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      Removal of flatulence causing sugars in soymilk

      Rajan, Anila
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      Rajan, A. (2009). Removal of flatulence causing sugars in soymilk (Thesis, Master of Engineering (ME)). The University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/3604
      Permanent Research Commons link: https://hdl.handle.net/10289/3604
      Abstract
      The enzyme, alpha-galactosidase was extracted from peanuts using various buffers

      and solvents. The specific activity of soluble preparation of peanut ranged from

      0.0083 to 0.0606 U/mg. Phosphate buffer extract showed the maximum specific

      activity of 0.0606 U/mg. The soluble alpha-galactosidase was immobilised in calcium

      alginate beads and the specific activity of the immobilized enzyme was found to

      be 0.0808 U/mg. Activity yield was 33%. The immobilised enzyme showed

      increase in activity compared with the soluble enzyme above 35 C. The thermal

      stability of the immobilised alpha-galactosidase was significantly improved in

      comparison to the soluble form. The effect of different pH depicts that at acidic

      pH the activity of the immobilised alpha-galactosidase was higher than that of the

      soluble enzyme. The Km and Vmax for immobilised enzyme was higher when

      compared with the soluble enzyme. Immobilised alpha-galactosidase retained 20%

      activity after 7 repeated uses. The immobilised enzyme exhibited high storage

      stability. The immobilised enzyme was used in batch and continuous packed bed

      reactors for the hydrolysis of stachyose and raffinose in soymilk. Flatulence

      causing raffinose and stachyose, after hydrolysis, was removed to a remarkable

      extent. Hydrolysis of flatulence causing oligosaccharides of soymilk in a

      continuous packed bed reactor has not been attempted previously.
      Date
      2009
      Type
      Thesis
      Degree Name
      Master of Engineering (ME)
      Publisher
      The University of Waikato
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