Show simple item record  

dc.contributor.authorRajan, Anilaen_NZ
dc.date.accessioned2010-02-16T20:41:43Z
dc.date.available2010-02-16T20:41:43Z
dc.date.issued2009en_NZ
dc.identifier.citationRajan, A. (2009). Removal of flatulence causing sugars in soymilk (Thesis, Master of Engineering (ME)). The University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/3604en
dc.identifier.urihttps://hdl.handle.net/10289/3604
dc.description.abstractThe enzyme, alpha-galactosidase was extracted from peanuts using various buffers and solvents. The specific activity of soluble preparation of peanut ranged from 0.0083 to 0.0606 U/mg. Phosphate buffer extract showed the maximum specific activity of 0.0606 U/mg. The soluble alpha-galactosidase was immobilised in calcium alginate beads and the specific activity of the immobilized enzyme was found to be 0.0808 U/mg. Activity yield was 33%. The immobilised enzyme showed increase in activity compared with the soluble enzyme above 35 C. The thermal stability of the immobilised alpha-galactosidase was significantly improved in comparison to the soluble form. The effect of different pH depicts that at acidic pH the activity of the immobilised alpha-galactosidase was higher than that of the soluble enzyme. The Km and Vmax for immobilised enzyme was higher when compared with the soluble enzyme. Immobilised alpha-galactosidase retained 20% activity after 7 repeated uses. The immobilised enzyme exhibited high storage stability. The immobilised enzyme was used in batch and continuous packed bed reactors for the hydrolysis of stachyose and raffinose in soymilk. Flatulence causing raffinose and stachyose, after hydrolysis, was removed to a remarkable extent. Hydrolysis of flatulence causing oligosaccharides of soymilk in a continuous packed bed reactor has not been attempted previously.en_NZ
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherThe University of Waikatoen_NZ
dc.rightsAll items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectAlpha -galactosidaseen_NZ
dc.subjectimmobilisationen_NZ
dc.subjectraffinoseen_NZ
dc.subjectstachyoseen_NZ
dc.subjectsoymilken_NZ
dc.titleRemoval of flatulence causing sugars in soymilken_NZ
dc.typeThesisen_NZ
thesis.degree.disciplineScience and Engineeringen_NZ
thesis.degree.grantorUniversity of Waikatoen_NZ
thesis.degree.levelMasters
thesis.degree.nameMaster of Engineering (ME)en_NZ
uow.date.accession2009-08-31T13:44:01Zen_NZ
uow.identifier.adthttp://adt.waikato.ac.nz/public/adt-uow20090831.134401
pubs.place-of-publicationHamilton, New Zealanden_NZ


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record