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dc.contributor.advisorHarfoot, C. G.
dc.contributor.authorRawley, Brian Andrew
dc.date.accessioned2013-11-25T02:22:49Z
dc.date.available2013-11-25T02:22:49Z
dc.date.issued1982
dc.identifier.citationRawley, B. A. (1982). Diurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes (Thesis, Master of Science (MSc)). University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/8228en
dc.identifier.urihttps://hdl.handle.net/10289/8228
dc.description.abstractA study was made of diurnal changes in the heterotrophic uptake kinetics of both ¹⁴C-glucose and ¹⁴C- glycolate in two small stratified lakes, Lake Rotomanuka in New Zealand and Lake Fryxell in Antarctica. The purpose of the investigation was to study the effect that diurnal changes in the extracellular release of certain substrates by algae might have on the uptake of those substrates by aquatic microheterotrophs. The heterotrophic uptake technique of Parsons and Strickland.(196l) was modified to provide a technique which was more convenient for field-work. In an effort to exclude the effects of migration and heterogeneous population distribution on substrate uptake, the microbial population in each lake was observed using a combination of (a) direct counts of bacteria (using acridine orange staining and epi-fluorescent microscopy); (b) measurement of chlorophyll α and bacterio-chlorophyll and (c) measurement of the reduction of INT dye. The natural concentration of glycolate was measured on some occasions using a modification of the technique of Shah and Wright (1974).
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherUniversity of Waikato
dc.rightsAll items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectAntarctica
dc.titleDiurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes
dc.typeThesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Waikato
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (MSc)
dc.date.updated2013-11-24T22:38:56Z
pubs.place-of-publicationHamilton, New Zealanden_NZ


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