Research Commons
      • Browse 
        • Communities & Collections
        • Titles
        • Authors
        • By Issue Date
        • Subjects
        • Types
        • Series
      • Help 
        • About
        • Collection Policy
        • OA Mandate Guidelines
        • Guidelines FAQ
        • Contact Us
      • My Account 
        • Sign In
        • Register
      View Item 
      •   Research Commons
      • University of Waikato Theses
      • Masters Degree Theses
      • View Item
      •   Research Commons
      • University of Waikato Theses
      • Masters Degree Theses
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      Applications of Hydroxymethylphosphines

      Wyllie, Megan Jane
      Thumbnail
      Files
      thesis.pdf
      8.956Mb
      Citation
      Export citation
      Wyllie, M. J. (2015). Applications of Hydroxymethylphosphines (Thesis, Master of Science (MSc)). University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/9604
      Permanent Research Commons link: https://hdl.handle.net/10289/9604
      Abstract
      Hydroxymethylphosphines are compounds containing P-CH₂-OH functional groups. They have numerous applications, of which three types were explored in this project.

      The cross-linking ability of tris(hydroxymethyl)phosphine (THP) was investigated for use as a fixative for biological samples for visualisation by scanning electron microscopy (SEM). THP has similar cross-linking ability to glutaraldehyde, the most commonly used fixative for SEM samples. Samples fixed with THP were compared with samples fixed with glutaraldehyde and an unfixed sample, under the SEM. THP appears to have a similar effect on the preservation of the samples, and seems to be successful as a fixative.

      The bonding between Ph₂P(S)CH₂OH and diiodine was investigated. The resulting adduct was unable to be crystallised. It was partially characterised by ³¹P NMR and IR spectroscopy. The related compound, Ph₃PS.I₂ was synthesised successfully and characterised by ³¹P NMR and IR spectroscopy.

      The ability of THP to immobilise compounds to solid supports, in the interest of immobilising fluorescent compounds was investigated. THP was used to immobilise N-(2-aminoethyl)-1,8-naphthalimide onto aminopropyl silica, and analysed under UV light. The resulting solid however, fluoresced only slightly. The reaction between Ph₂P(CH₂OH) ₂Cl and N-(2-aminoethyl)-1,8-naphthalimide was also investigated, but the product was unable to be crystallised.
      Date
      2015
      Type
      Thesis
      Degree Name
      Master of Science (MSc)
      Supervisors
      Henderson, William
      Publisher
      University of Waikato
      Rights
      All items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
      Collections
      • Masters Degree Theses [2385]
      Show full item record  

      Usage

      Downloads, last 12 months
      27
       
       

      Usage Statistics

      For this itemFor all of Research Commons

      The University of Waikato - Te Whare Wānanga o WaikatoFeedback and RequestsCopyright and Legal Statement