RAPD Analysis of East Antarctic Pottia heimii Populations

Dale, Tracy Maree

RAPD Analysis of East Antarctic Pottia heimii Populations

dc.contributor.advisor	Skotnicki, Mary
dc.contributor.author	Dale, Tracy Maree
dc.date.accessioned	2019-09-05T01:38:58Z
dc.date.available	2019-09-05T01:38:58Z
dc.date.issued	1997
dc.date.updated	2019-09-05T01:30:36Z
dc.description.abstract	Pottia heimii contributes significantly to the sparse terrestrial vegetation in East Antarctica, especially within the Dry Valley region. Using Randomly Amplified Polymorphic DNA (RAPD), levels of genetic variation were investigated in several East Antarctic P. heimii populations. The initial stage of the study optimised the RAPD protocol specifically for antarctic P. heimii. RAPDs gave similar profiles for genetically identical P. heimii tissue (shoots joined at the base) and were able to detect DNA differences between individual shoots within a moss clump, confirming the usefulness of RAPDs for P. heimii population studies. Genetic distance matrices calculated from RAPD banding patterns were presented as dendrograms and used in an Analysis of Molecular Variance (AMOV A). Using single moss shoots, a significant level of genetic variation was detected within three 50 metre transects collected in the Miers Valley. The genetic spatial structure within these transects was random indicated by the distribution of genotypes. This suggested wind was the most likely agent for local propagule dispersal. No genetic subdivision existed between the transects, therefore individuals could be considered to be taken from a single population. Comparison of individuals from Miers Valley, East Garwood Valley, Bratina Island and Edmonson Point showed the first three Southern Victoria Land populations could be considered as a single larger population genetically distinct from the more northerly Edmonson Point population. The level of genetic variation within all the East Antarctic P. heimii populations analysed was considered significant.
dc.format.mimetype	application/pdf
dc.identifier.citation	Dale, T. M. (1997). RAPD Analysis of East Antarctic Pottia heimii Populations (Thesis, Master of Science (MSc)). The University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/12829	en
dc.identifier.uri	https://hdl.handle.net/10289/12829
dc.language.iso	en
dc.publisher	The University of Waikato
dc.rights	All items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.title	RAPD Analysis of East Antarctic Pottia heimii Populations
dc.type	Thesis
pubs.place-of-publication	Hamilton, New Zealand	en_NZ
thesis.degree.grantor	The University of Waikato
thesis.degree.level	Masters
thesis.degree.name	Master of Science (MSc)